Carvalho V T, Gomes R T, Viotti A P, Freitas T V
Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias (FUNED), Belo Horizonte, Brazil.
Toxicon. 2000 Jun;38(6):881-6. doi: 10.1016/s0041-0101(99)00201-9.
The venom of Bothrops jararaca (BjV) snake was encapsulated into liposomes. The toxicity and ability of the resultant liposomes to protect mice were evaluated. No acute toxicity was found in mice, when liposomes were subcutaneously injected whereas the same dose of venom emulsified in Freund's adjuvant caused the mice to die. Immunization with the venom containing liposomes and associated immunostimulants, protected 50% of mice after challenge with BjV (4 x LD50). The hemorrhagic activity induced by BjV was reduced after immunization with liposomes associated with immunostimulants, similarly to immunization with BjV emulsified in Freund's adjuvant. However, mice immunized with BjV:Freund's were better protected against the lethal effects of the venom.
矛头蝮蛇毒（BjV）被包裹于脂质体中。对所得脂质体的毒性及保护小鼠的能力进行了评估。当脂质体皮下注射时，未在小鼠中发现急性毒性，而相同剂量的毒液乳化于弗氏佐剂中则导致小鼠死亡。用含毒液的脂质体及相关免疫刺激剂进行免疫后，在用矛头蝮蛇毒（4倍半数致死量）攻击后，50%的小鼠得到保护。与免疫刺激剂相关的脂质体免疫后，矛头蝮蛇毒诱导的出血活性降低，这与用乳化于弗氏佐剂中的矛头蝮蛇毒免疫相似。然而，用矛头蝮蛇毒与弗氏佐剂免疫的小鼠对毒液的致死作用具有更好的保护作用。
