Liu X, Peng D, Wu X, Xing L, Zhang R
Department of Veterinary Science, Yangzhou University, Jiangsu, P.R. China.
Acta Virol. 1999 Apr-Jun;43(2-3):201-4.
Recombinant fowlpox virus (rFPV) was constructed to express glycoprotein B (gB) gene from CVI988/Rispens strain of Marek's disease virus (MDV). The rFPV-gB/R alone and in combination with herpesvirus of turkey (HVT) preparations were evaluated for their protective efficacy against challenge with very virulent MDV strains Md5 and RB1B in different chickens. The rFPV-gB/R alone induced protection comparable to that by HVT vaccines in both Ab- SPF chickens and Ab+ production chickens. Significant protective synergism was observed in one of these two types of commercial production chickens when rFPV-gB/R was combined with HVT of either cell-associated or cell-free preparations. Immunogenesis studies showed that rFPV-gB/R, just like conventional vaccines, significantly reduced the level of viremia, splenocytes infection and feather follicle shedding of challenge virus in vaccinated chickens.
构建了重组禽痘病毒（rFPV）以表达来自马立克氏病病毒（MDV）CVI988/Rispens株的糖蛋白B（gB）基因。单独评估了rFPV-gB/R以及与火鸡疱疹病毒（HVT）制剂联合使用时，在不同鸡群中对超强毒MDV毒株Md5和RB1B攻击的保护效果。单独的rFPV-gB/R在抗体阴性的SPF鸡和抗体阳性的商品鸡中诱导的保护效果与HVT疫苗相当。当rFPV-gB/R与细胞相关或无细胞制剂的HVT联合使用时，在这两种类型的商品鸡中的一种中观察到了显著的保护协同作用。免疫发生学研究表明，rFPV-gB/R与传统疫苗一样，显著降低了接种疫苗鸡的病毒血症水平、脾细胞感染和攻击病毒的毛囊脱落。
