Nemcová V, Petrovický P, ten Donkelaar H J
Anatomical Institute of the First Medical Faculty, Charles University, Prague, Czech Republic.
J Chem Neuroanat. 2000 Jan;17(4):227-32. doi: 10.1016/s0891-0618(99)00053-8.
Cholinergic neurons of the mesopontine complex have extensive ascending projections to the forebrain: the laterodorsal tegmental nucleus extensively innervates the anterior thalamus, the anteroventral nucleus in particular, whereas the pedunculopontine nucleus has widespread projections to both the thalamus and extrapyramidal structures. Most of their neurons express nitric oxide synthase (NOS) activity. Following electrolytic lesions of the anteroventral thalamic nucleus, nicotinamide adenine dinucleotide phosphate-diaphorase (NADPHd) activity in neurons of the laterodorsal tegmental nucleus changed drastically. The intensity of NADPH-diaphorase staining increased in laterodorsal tegmental neurons ipsilateral to the lesion side, but decreased contralaterally. The intensity of the NADPH-diaphorase staining of neurons of the pedunculopontine nucleus, however, remained unchanged bilaterally. After partial lesions of the anteroventral thalamic nucleus a similar effect was noted. In contrast, large electrolytic lesions involving other thalamic nuclei or extrapyramidal structures did not change the number of NADPH-diaphorase neurons or their intensity of staining in the laterodorsal tegmental nuclei. These data show that electrolytic lesions of target areas can lead to an upregulation of NOS expression in the parent cell bodies, provided that there is no wide collateralization as found for the pedunculopontine nucleus.
外侧背侧被盖核广泛支配前丘脑，特别是前腹侧核，而脚桥被盖核则广泛投射到丘脑和锥体外系结构。它们的大多数神经元表达一氧化氮合酶（NOS）活性。在前腹侧丘脑核进行电解损伤后，外侧背侧被盖核神经元中的烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPHd）活性发生了显著变化。损伤侧同侧的外侧背侧被盖神经元中NADPH-黄递酶染色强度增加，而对侧则降低。然而，脚桥被盖核神经元的NADPH-黄递酶染色强度在双侧均保持不变。在前腹侧丘脑核部分损伤后也观察到类似的效应。相比之下，涉及其他丘脑核或锥体外系结构的大的电解损伤并未改变外侧背侧被盖核中NADPH-黄递酶神经元的数量或其染色强度。这些数据表明，目标区域的电解损伤可导致母细胞体中NOS表达上调，前提是不存在像脚桥被盖核那样广泛的侧支联系。
