Hołownia A, Kita J, Chyczewski L, Kozłowski M, Braszko J J
Clinical Pharmacology Unit, Medical Academy of Bialystok.
Rocz Akad Med Bialymst. 1999;44:180-9.
The aim of this study was answering the question whether cytofluorimetric determining of DNA ploidy, p53 and bcl-2 expression in tumour cells may enhance the diagnosis of squamous epithelial lung carcinoma. Samples of neoplastic tissue were taken from 11 operated patients. DNA staining was performed with propidium iodide. p53 and bcl-2 expression was evaluated by using monoclonal antibodies. By assessing of DNA ploidy we observed increased proliferative activity and, at the same time, high rate of the cell necrosis. In the analysis of p53 protein the presence of antigen was observed on average in 7.1 +/- 2.4, 15.1 +/- 3.9, and 19.8 +/- 4.1% of normal cells of the pulmonary tissue, the peripheral part of the tumour and cells of the central part of the tumour, respectively. bcl-2 was found on average in 7.3 +/- 2.2, 9.9 +/- 3.1, and 12.4 +/- 3.19% of the above cells, respectively. These results show that the technique of cytofluorimetric evaluation of p53 and bcl-2 proteins can be useful in diagnosis of pulmonary cancer in addition to classic histological and immunohistochemical methods.
本研究的目的是回答肿瘤细胞中DNA倍体、p53和bcl-2表达的细胞荧光测定是否可提高肺鳞状上皮癌的诊断水平。从11例接受手术的患者身上获取肿瘤组织样本。用碘化丙啶进行DNA染色。使用单克隆抗体评估p53和bcl-2的表达。通过评估DNA倍体，我们观察到增殖活性增加，同时细胞坏死率较高。在对p53蛋白的分析中，在肺组织正常细胞、肿瘤周边部分细胞和肿瘤中央部分细胞中，抗原的平均存在率分别为7.1±2.4%、15.1±3.9%和19.8±4.1%。bcl-2在上述细胞中的平均发现率分别为7.3±2.2%、9.9±3.1%和12.4±3.19%。这些结果表明，除了经典的组织学和免疫组织化学方法外，p53和bcl-2蛋白的细胞荧光评估技术可用于肺癌的诊断。
