Chinnici J P, Erlanger L, Charnock M, Jones M, Stein J
Chem Biol Interact. 1979 Mar;24(3):373-80. doi: 10.1016/0009-2797(79)90084-x.
Using Drosophila melanogaster, the relative sensitivities of various larval stages to the toxic effects of growth on media supplemented with either 0.44 or 0.88 ppm aflatoxin B1 (AFB1) were determined. Two strains of fruit flies were tested: strain A-11 which is relatively resistant to AFB1 induced toxicity, and strain A-9 which is quite sensitive. Eggs, mid-first, mid-second and early-, mid- and late-third instar larvae were transferred onto AFB1 media and allowed to complete larval and pupal development and eclose as adults. At the 0.44 ppm concentration, strain A-11 showed no effect, while only first instar larvae of strain A-9 showed significant mortality rates for first instar larvae, but the A-9 larvae die at higher rates than the A-11 larvae. In addition, second and third instar larvae of strain A-9 show significant mortality rates when grown at 0.88 ppm AFB1, while these stages are not affected in strain A-11.
利用黑腹果蝇，测定了不同幼虫阶段对添加0.44或0.88 ppm黄曲霉毒素B1（AFB1）的培养基上生长的毒性作用的相对敏感性。测试了两种果蝇品系：对AFB1诱导的毒性相对抗性的A-11品系，以及相当敏感的A-9品系。将卵、一龄中期、二龄中期和三龄早期、中期和晚期幼虫转移到含有AFB1的培养基上，使其完成幼虫和蛹的发育并羽化为成虫。在0.44 ppm浓度下，A-11品系没有表现出影响，而A-9品系只有一龄幼虫表现出显著的死亡率，但A-9幼虫的死亡率高于A-11幼虫。此外，当在0.88 ppm AFB1下生长时，A-9品系的二龄和三龄幼虫表现出显著的死亡率，而这些阶段在A-11品系中不受影响。
