Wilson T G, Cryan J R
Department of Biology, Colorado State University, Fort Collins 80523, USA.
J Exp Zool. 1997 May 1;278(1):37-44. doi: 10.1002/(sici)1097-010x(19970501)278:1<37::aid-jez4>3.0.co;2-7.
The chitin-synthesis inhibitor lufenuron was administered to Drosophila melanogaster to better understand the effects of chitin-synthesis interruption during the development and reproduction of this insect. When larvae were fed a diet containing a low concentration (< 1 ppm) of lufenuron, mortality was observed during either larval or pupal development, depending on the dose. Survivor adults were unable to fly, probably due to abnormal cuticular development in the wing hinge regions of the thorax. Larvae fed a higher concentration (10 ppm) completed development within that instar, but died during ecdysis to the next instar, presumably due to inadequate cuticle synthesis. Third instar larvae pupariated, but the puparium was abnormal, and pupation did not occur. Adults fed 10 ppm showed normal viability but slightly depressed oogenesis; additionally, although their eggs were fertilized, they failed to hatch. Examination of the embryos showed that they completed development but were unable to perforate the surrounding vitelline membrane, probably due to a weakened chitinous mouth hook assembly that was insufficiently rigid to effect hatching. These results identify stages during D. melanogaster development when chitin synthesis and deposition are critical. This information will be useful for identifying chitin-synthesis mutants of this insect.
向黑腹果蝇施用几丁质合成抑制剂虱螨脲，以更好地了解几丁质合成中断对该昆虫发育和繁殖的影响。当给幼虫喂食含有低浓度（<1 ppm）虱螨脲的食物时，根据剂量不同，在幼虫或蛹发育阶段会观察到死亡情况。存活下来的成虫无法飞行，这可能是由于胸部翅铰链区域的表皮发育异常所致。喂食较高浓度（10 ppm）虱螨脲的幼虫在该龄期内完成发育，但在蜕皮进入下一龄期时死亡，推测是由于表皮合成不足。三龄幼虫化蛹，但蛹壳异常，无法完成化蛹。喂食10 ppm虱螨脲的成虫具有正常的活力，但卵子发生略有抑制；此外，尽管它们的卵已受精，但未能孵化。对胚胎的检查表明，它们完成了发育，但无法穿透周围的卵黄膜，这可能是由于几丁质口钩组装体变弱，其刚性不足以实现孵化。这些结果确定了黑腹果蝇发育过程中几丁质合成和沉积至关重要的阶段。这些信息将有助于鉴定该昆虫的几丁质合成突变体。
