Kisiel D, Gindzieński A, Gadek A
Department of General and Organic Chemistry, Institute of Chemistry, Medical Academy of Białystok, Poland.
Acta Biochim Pol. 1999;46(3):753-7.
O-Glycans from pig gastric mucin were released by beta-elimination in 0.2 M triethylamine and 50% aqueous hydrazine solution. The released glycan hydrazides were isolated using Centricon 10 separators, brought to their reducing form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABEE). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.
猪胃粘蛋白中的O-聚糖在0.2M三乙胺和50%水合肼溶液中通过β-消除反应释放出来。使用Centricon 10分离器分离释放出的聚糖酰肼，将其还原成还原形式，并用对氨基苯甲酸乙酯（ABEE）进行还原性标记。标记产物在Bio-Gel P4柱上分离为中性和酸性组分，该柱用葡聚糖寡糖混合物校准，按照相同程序进行标记。
