Coutts M, Zou X, Calame K
Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
Oncogene. 2000 Feb 10;19(6):801-9. doi: 10.1038/sj.onc.1203398.
Transformation of 3T3 fibroblasts by the v-Abl tyrosine kinase replaces mitogenic and adhesion signals normally required for cell cycle progression. A 3T3 cell line conditionally transformed with v-Abl has been used to study v-Abl's effects on cell cycle in the context of either serum depletion or absence of adhesion signals. We show that E2F-dependent mRNAs, encoding proteins required for cell cycle progression, are induced by v-Abl. In addition, we identify two previously unknown targets of v-Abl signaling: (1) cyclin D1 and D2 mRNAs are induced upon v-Abl activation; and (2) the CDK inhibitor p27 is decreased upon v-Abl activation.
v-Abl酪氨酸激酶对3T3成纤维细胞的转化取代了细胞周期进程中正常所需的促有丝分裂和黏附信号。一种用v-Abl进行条件性转化的3T3细胞系已被用于研究在血清缺乏或缺乏黏附信号的情况下v-Abl对细胞周期的影响。我们发现，v-Abl可诱导依赖E2F的mRNA，这些mRNA编码细胞周期进程所需的蛋白质。此外，我们确定了v-Abl信号传导的两个先前未知的靶点：（1）v-Abl激活后可诱导细胞周期蛋白D1和D2的mRNA；（2）v-Abl激活后CDK抑制剂p27减少。
