El-Yazbi F A, Abdine H H, Shaalan R A
Faculty of Pharmacy, Alexandria University, El-Mesallah, Egypt.
J Pharm Biomed Anal. 1999 May;19(6):819-27. doi: 10.1016/s0731-7085(98)00110-1.
Simple and sensitive methods are described for the assay of lisinopril in tablets. The first method (A) is based on the reaction of the drug with chloranil in aqueous solution of pH 9.5 to give yellow colour measured at 346 nm. The second method (B) is based upon the interaction of lisinopril with dichlone resulting in the formation of an intense purple colour measured at 580 nm. The third method (C) depends on the reaction of the drug with acetylacetone and formaldehyde to form a coloured condensation product measured at 356 nm and also has a strong fluorescence at 475 nm (lambda(ex) 410 nm). This method is extended to determine lisinopril in binary mixtures with hydrochlorothiazide. The last method (D) depends on measuring the first and second derivative spectra of lisinopril. Moreover, the derivative method is used as stability-indicating method where lisinopril can be determined in presence of its degradation products. The proposed methods proved to be suitable for a rapid quality control of commercial dosage forms. The results obtained were precise and accurate.
本文描述了用于测定片剂中赖诺普利的简单且灵敏的方法。第一种方法（A）基于药物在pH 9.5的水溶液中与四氯苯醌反应，产生黄色，于346 nm处测定。第二种方法（B）基于赖诺普利与二氯萘醌的相互作用，形成强烈的紫色，于580 nm处测定。第三种方法（C）取决于药物与乙酰丙酮和甲醛反应形成有色缩合产物，于356 nm处测定，且在475 nm（激发波长410 nm）处有强荧光。该方法扩展用于测定与氢氯噻嗪的二元混合物中的赖诺普利。最后一种方法（D）取决于测量赖诺普利的一阶和二阶导数光谱。此外，导数法用作稳定性指示方法，可在其降解产物存在的情况下测定赖诺普利。所提出的方法被证明适用于商业剂型的快速质量控制。获得的结果精确且准确。
