LaCourse W R, Dasenbrock C O
Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore 21250, USA.
J Pharm Biomed Anal. 1999 Feb;19(1-2):239-52. doi: 10.1016/s0731-7085(98)00089-2.
Pulsed electrochemical detection (PED) following reversed-phase chromatography has been applied to the direct detection of sulfur-containing antibiotics, specifically, penicillins, cephalosporins, and lincomycin. The compounds are detected sensitively and selectively without the need for derivatization. Integrated pulsed amperometric detection (IPAD) yields limits of detection lower than UV detection for these compounds. Detection limits using an optimized IPAD waveform are typically 10 ppb or less. The high selectivity of PED for thiocompounds reduces sample preparation. This work is applied to the determination of penicillin and related analogues in various pharmaceutical formulations/preparations, including a chicken feed.
反相色谱后的脉冲电化学检测（PED）已应用于含硫抗生素的直接检测，具体而言，包括青霉素、头孢菌素和林可霉素。这些化合物无需衍生化即可灵敏且选择性地检测。对于这些化合物，集成脉冲安培检测（IPAD）的检测限低于紫外检测。使用优化的IPAD波形时，检测限通常为10 ppb或更低。PED对硫化合物的高选择性减少了样品制备。这项工作应用于各种药物制剂/制品中青霉素及相关类似物的测定，包括鸡饲料。
