氧化亚铁硫杆菌中亚铁离子或硫氧化的选择性抑制
Selective inhibition of the oxidation of ferrous iron or sulfur in Thiobacillus ferrooxidans.
作者信息
Harahuc L, Lizama H M, Suzuki I
机构信息
Department of Microbiology, University of Manitoba, Winnipeg, Manitoba.
出版信息
Appl Environ Microbiol. 2000 Mar;66(3):1031-7. doi: 10.1128/AEM.66.3.1031-1037.2000.
The oxidation of either ferrous iron or sulfur by Thiobacillus ferrooxidans was selectively inhibited or controlled by various anions, inhibitors, and osmotic pressure. Iron oxidation was more sensitive than sulfur oxidation to inhibition by chloride, phosphate, and nitrate at low concentrations (below 0.1 M) and also to inhibition by azide and cyanide. Sulfur oxidation was more sensitive than iron oxidation to the inhibitory effect of high osmotic pressure. These differences were evident not only between iron oxidation by iron-grown cells and sulfur oxidation by sulfur-grown cells but also between the iron and sulfur oxidation activities of the same iron-grown cells. Growth experiments with ferrous iron or sulfur as an oxidizable substrate confirmed the higher sensitivity of iron oxidation to inhibition by phosphate, chloride, azide, and cyanide. Sulfur oxidation was actually stimulated by 50 mM phosphate or chloride. Leaching of Fe and Zn from pyrite (FeS(2)) and sphalerite (ZnS) by T. ferrooxidans was differentially affected by phosphate and chloride, which inhibited the solubilization of Fe without significantly affecting the solubilization of Zn.
氧化亚铁硫杆菌对亚铁离子或硫的氧化作用可被各种阴离子、抑制剂和渗透压选择性抑制或控制。在低浓度（低于0.1M）时，亚铁离子氧化比硫氧化对氯化物、磷酸盐和硝酸盐的抑制更敏感，对叠氮化物和氰化物的抑制也更敏感。硫氧化比亚铁离子氧化对高渗透压的抑制作用更敏感。这些差异不仅在以铁生长的细胞进行的铁氧化和以硫生长的细胞进行的硫氧化之间明显，而且在相同的以铁生长的细胞的铁和硫氧化活性之间也很明显。以亚铁离子或硫作为可氧化底物的生长实验证实了铁氧化对磷酸盐、氯化物、叠氮化物和氰化物抑制的更高敏感性。硫氧化实际上受到50mM磷酸盐或氯化物的刺激。氧化亚铁硫杆菌对黄铁矿（FeS₂）和闪锌矿（ZnS）中Fe和Zn的浸出受到磷酸盐和氯化物的不同影响，它们抑制了Fe的溶解，而对Zn的溶解没有显著影响。
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