Waché Y, Laroche C, Bergmark K, Møller-Andersen C, Aguedo M, Le Dall M T, Wang H, Nicaud J M, Belin J M
Laboratoire de Biotechnologie, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Université de Bourgogne, 21000 Dijon, France.
Appl Environ Microbiol. 2000 Mar;66(3):1233-6. doi: 10.1128/AEM.66.3.1233-1236.2000.
We reported previously on the function of acyl coenzyme A (acyl-CoA) oxidase isozymes in the yeast Yarrowia lipolytica by investigating strains disrupted in one or several acyl-CoA oxidase-encoding genes (POX1 through POX5) (H. Wang et al., J. Bacteriol. 181:5140-5148, 1999). Here, these mutants were studied for lactone production. Monodisrupted strains produced similar levels of lactone as the wild-type strain (50 mg/liter) except for Deltapox3, which produced 220 mg of gamma-decalactone per liter after 24 h. The Deltapox2 Deltapox3 double-disrupted strain, although slightly affected in growth, produced about 150 mg of lactone per liter, indicating that Aox2p was not essential for the biotransformation. The Deltapox2 Deltapox3 Deltapox5 triple-disrupted strain produced and consumed lactone very slowly. On the contrary, the Deltapox2 Deltapox3 Deltapox4 Deltapox5 multidisrupted strain did not grow or biotransform methyl ricinoleate into gamma-decalactone, demonstrating that Aox4p is essential for the biotransformation.
我们之前通过研究破坏了一个或几个酰基辅酶A（acyl-CoA）氧化酶编码基因（POX1至POX5）的酵母解脂耶氏酵母菌株，报道了酰基辅酶A氧化酶同工酶的功能（H. Wang等人，《细菌学杂志》181:5140 - 5148，1999年）。在此，对这些突变体进行了内酯生产研究。单基因破坏的菌株产生的内酯水平与野生型菌株相似（50毫克/升），除了Δpox3，其在24小时后每升产生220毫克γ-癸内酯。Δpox2Δpox3双基因破坏的菌株虽然生长略有受影响，但每升产生约150毫克内酯，表明Aox2p对于生物转化不是必需的。Δpox2Δpox3Δpox5三基因破坏的菌株产生和消耗内酯非常缓慢。相反，Δpox2Δpox3Δpox4Δpox5多基因破坏的菌株不生长，也不能将蓖麻油酸甲酯生物转化为γ-癸内酯，表明Aox4p对于生物转化是必需的。
