烟草花叶病毒运动蛋白与宿主细胞果胶甲酯酶之间的相互作用是病毒细胞间运动所必需的。
Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell-to-cell movement.
作者信息
Chen M H, Sheng J, Hind G, Handa A K, Citovsky V
机构信息
Department of Biochemistry, State University of New York, Stony Brook, NY 11794-5215, USA.
出版信息
EMBO J. 2000 Mar 1;19(5):913-20. doi: 10.1093/emboj/19.5.913.
Abstract
Virus-encoded movement protein (MP) mediates cell-to-cell spread of tobacco mosaic virus (TMV) through plant intercellular connections, the plasmodesmata. The molecular pathway by which TMV MP interacts with the host cell is largely unknown. To understand this process better, a cell wall-associated protein that specifically binds the viral MP was purified from tobacco leaf cell walls and identified as pectin methylesterase (PME). In addition to TMV MP, PME is recognized by MPs of turnip vein clearing virus (TVCV) and cauliflower mosaic virus (CaMV). The use of amino acid deletion mutants of TMV MP showed that its domain was necessary and sufficient for association with PME. Deletion of the PME-binding region resulted in inactivation of TMV cell-to-cell movement.
摘要
病毒编码的运动蛋白（MP）通过植物细胞间连接——胞间连丝介导烟草花叶病毒（TMV）在细胞间的传播。TMV MP与宿主细胞相互作用的分子途径在很大程度上尚不清楚。为了更好地理解这一过程，从烟草叶细胞壁中纯化出一种能特异性结合病毒MP的细胞壁相关蛋白，并鉴定为果胶甲基酯酶（PME）。除了TMV MP外，芜菁叶脉明脉病毒（TVCV）和花椰菜花叶病毒（CaMV）的MP也能识别PME。使用TMV MP的氨基酸缺失突变体表明，其结构域对于与PME的结合是必要且充分的。删除PME结合区域会导致TMV细胞间运动失活。
相似文献
1
Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell-to-cell movement.烟草花叶病毒运动蛋白与宿主细胞果胶甲酯酶之间的相互作用是病毒细胞间运动所必需的。
EMBO J. 2000 Mar 1;19(5):913-20. doi: 10.1093/emboj/19.5.913.
2
A novel function for a ubiquitous plant enzyme pectin methylesterase: the host-cell receptor for the tobacco mosaic virus movement protein.一种普遍存在的植物酶——果胶甲酯酶的新功能：烟草花叶病毒运动蛋白的宿主细胞受体。
FEBS Lett. 1999 Nov 19;461(3):223-8. doi: 10.1016/s0014-5793(99)01447-7.
3
How do pectin methylesterases and their inhibitors affect the spreading of tobamovirus?果胶甲酯酶及其抑制剂如何影响烟草花叶病毒的扩散？
Plant Signal Behav. 2014;9(12):e972863. doi: 10.4161/15592316.2014.972863.
4
The Plasmodesmal Localization Signal of TMV MP Is Recognized by Plant Synaptotagmin SYTA.TMV MP 的质膜定位信号由植物突触结合蛋白 SYTA 识别。
mBio. 2018 Jul 10;9(4):e01314-18. doi: 10.1128/mBio.01314-18.
5
Transgenic expression of pectin methylesterase inhibitors limits tobamovirus spread in tobacco and Arabidopsis.果胶甲酯酶抑制剂的转基因表达限制烟草花叶病毒在烟草和拟南芥中的传播。
Mol Plant Pathol. 2014 Apr;15(3):265-74. doi: 10.1111/mpp.12090. Epub 2013 Dec 5.
6
Limitations to tobacco mosaic virus infection of turnip.芜菁对烟草花叶病毒感染的局限性。
Arch Virol. 1999;144(5):957-71. doi: 10.1007/s007050050558.
7
Tobacco mosaic virus movement protein-mediated protein transport between trichome cells.烟草花叶病毒运动蛋白介导的毛状体细胞间的蛋白质运输。
Plant Cell. 1995 Dec;7(12):2069-79. doi: 10.1105/tpc.7.12.2069.
8
ANK, a host cytoplasmic receptor for the Tobacco mosaic virus cell-to-cell movement protein, facilitates intercellular transport through plasmodesmata.ANK，一种烟草花叶病毒细胞间运动蛋白的宿主细胞质受体，通过胞间连丝促进细胞间运输。
PLoS Pathog. 2010 Nov 18;6(11):e1001201. doi: 10.1371/journal.ppat.1001201.
9
Tobacco mosaic virus (TMV) replicase and movement protein function synergistically in facilitating TMV spread by lateral diffusion in the plasmodesmal desmotubule of Nicotiana benthamiana.烟草花叶病毒（TMV）复制酶和运动蛋白在促进TMV通过在本氏烟草胞间连丝的连丝微管中侧向扩散来传播方面发挥协同作用。
Mol Plant Microbe Interact. 2008 Mar;21(3):335-45. doi: 10.1094/MPMI-21-3-0335.
10
Effects of calreticulin on viral cell-to-cell movement.钙网蛋白对病毒细胞间运动的影响。
Plant Physiol. 2005 Aug;138(4):1866-76. doi: 10.1104/pp.105.064386. Epub 2005 Jul 8.
引用本文的文献
1
Microscopic probing into plasmodesmata (PD) and PD-derived intercellular gateways (PdIGs): Beyond morphology.对胞间连丝（PD）和源自胞间连丝的细胞间通道（PdIGs）的微观探究：超越形态学
Protoplasma. 2025 Jul 11. doi: 10.1007/s00709-025-02088-4.
2
Viral tropism in plants, reproductive tissues, and seeds.病毒在植物、生殖组织和种子中的嗜性。
Arch Microbiol. 2025 May 23;207(7):152. doi: 10.1007/s00203-025-04353-9.
3
The roles of movement and coat proteins in the transport of tobamoviruses between plant cells.运动蛋白和外壳蛋白在烟草花叶病毒在植物细胞间运输中的作用。
Front Plant Sci. 2025 Apr 17;16:1580554. doi: 10.3389/fpls.2025.1580554. eCollection 2025.
4
Tobacco Mosaic Virus Movement: From Capsid Disassembly to Transport Through Plasmodesmata.烟草花叶病毒的移动：从衣壳解体到通过胞间连丝的运输
Viruses. 2025 Jan 31;17(2):214. doi: 10.3390/v17020214.
5
HSP90 interacts with VP37 to facilitate the cell-to-cell movement of broad bean wilt virus 2.热休克蛋白90（HSP90）与VP37相互作用，以促进蚕豆萎蔫病毒2在细胞间的移动。
mBio. 2025 Mar 12;16(3):e0250024. doi: 10.1128/mbio.02500-24. Epub 2025 Feb 19.
6
Cellular Partners of Tobamoviral Movement Proteins.烟草花叶病毒移动蛋白的细胞伴侣
Int J Mol Sci. 2025 Jan 5;26(1):400. doi: 10.3390/ijms26010400.
7
Five questions on the cell-to-cell movement of .关于……细胞间运动的五个问题。
你提供的原文“Five questions on the cell-to-cell movement of.”似乎不完整，后面缺少具体所指内容。
BBA Adv. 2024 Oct 16;6:100124. doi: 10.1016/j.bbadva.2024.100124. eCollection 2024.
8
Engineered Resistance to Tobamoviruses.工程化抗烟草花叶病毒。
Viruses. 2024 Jun 22;16(7):1007. doi: 10.3390/v16071007.
9
Homogalacturonan Pectins Tuned as an Effect of Susceptible rbohD, Col-0-Reactions, and Resistance rbohF-, rbohD/F-Reactions to TuMV.果胶同型半乳糖醛酸聚糖受 rbohD、Col-0 反应和 rbohF-、rbohD/F 反应的影响而变化，以抵御 TuMV。
Int J Mol Sci. 2024 May 11;25(10):5256. doi: 10.3390/ijms25105256.
10
A novel cellular factor of susceptibility to tobamovirus infection.一种对烟草花叶病毒感染易感性的新型细胞因子。
Front Plant Sci. 2023 Jul 18;14:1224958. doi: 10.3389/fpls.2023.1224958. eCollection 2023.
本文引用的文献
1
Localization by immunogold cytochemistry of the virus-coded 30K protein in plasmodesmata of leaves infected with tobacco mosaic virus.通过免疫金细胞化学对烟草花叶病毒感染叶片胞间连丝中病毒编码的30K蛋白进行定位。
Virology. 1987 Oct;160(2):363-71. doi: 10.1016/0042-6822(87)90007-9.
2
Role of Pectinesterase in pH-Dependent Interactions between Pea Cell Wall Polymers.果胶酯酶在豌豆细胞壁聚合物与 pH 值依赖性相互作用中的作用。
Plant Physiol. 1984 Oct;76(2):547-9. doi: 10.1104/pp.76.2.547.
3
Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit.烟草花叶病毒的运动蛋白可改变胞间连丝的大小排阻极限。
Science. 1989 Oct 20;246(4928):377-9. doi: 10.1126/science.246.4928.377.
4
TRANSPORT OF PROTEINS AND NUCLEIC ACIDS THROUGH PLASMODESMATA.蛋白质和核酸通过胞间连丝的运输
Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:27-50. doi: 10.1146/annurev.arplant.48.1.27.
5
The isolation of defective tobacco mosaic virus strains.有缺陷烟草花叶病毒株的分离
Proc Natl Acad Sci U S A. 1962 Oct 15;48(10):1845-51. doi: 10.1073/pnas.48.10.1845.
6
Cell-to-Cell and Long-Distance Transport of Viruses in Plants.植物中病毒的细胞间和长距离运输
Plant Cell. 1996 Oct;8(10):1669-1681. doi: 10.1105/tpc.8.10.1669.
7
Viral movement proteins as probes for intracellular and intercellular trafficking in plants.病毒运动蛋白作为植物细胞内和细胞间运输的探针
Plant Cell. 1999 Apr;11(4):535-48. doi: 10.1105/tpc.11.4.535.
8
Preservation of plant cell ultrastructure during immunolocalization of virus particles.病毒粒子免疫定位过程中植物细胞超微结构的保存
J Virol Methods. 1998 Oct;74(2):223-9. doi: 10.1016/s0166-0934(98)00084-6.
9
Inhibition of plant viral systemic infection by non-toxic concentrations of cadmium.无毒浓度的镉对植物病毒系统感染的抑制作用。
Plant J. 1998 Mar;13(5):591-602. doi: 10.1046/j.1365-313x.1998.00061.x.
10
Changing patterns of localization of the tobacco mosaic virus movement protein and replicase to the endoplasmic reticulum and microtubules during infection.烟草花叶病毒运动蛋白和复制酶在感染过程中定位于内质网和微管的模式变化。
Plant Cell. 1998 Jul;10(7):1107-20. doi: 10.1105/tpc.10.7.1107.
Zotero 插件