Tomenius K, Clapham D, Meshi T
Swedish University of Agricultural Sciences, Department of Plant and Forest Protection, P.O. Box 7044, S-750 07 Uppsala, Sweden.
Virology. 1987 Oct;160(2):363-71. doi: 10.1016/0042-6822(87)90007-9.
The 30K protein of tobacco mosaic virus (TMV) was localized to the plasmodesmata of infected tobacco leaves by immunogold cytochemistry. This protein has been reported to be in the nuclear fraction of TMV-infected protoplasts, but as it has been proposed to function in cell-to-cell transport of virus, probably via the plasmodesmata, intact tissue was investigated with particular attention directed to plasmodesmata and nuclei. Thin sections were made from leaves mechanically inoculated with TMV at different times. Affinity-purified antibodies against a synthetic peptide corresponding to the C-terminal sequence of the 30K protein were used in the incubations, and parallel sections were incubated with antibodies against TMV. The 30K protein label accumulated inside the plasmodesmata, with a maximum 24 hr after inoculation. No specific label was found in the nuclei or at any other site in the cells.
通过免疫金细胞化学方法，烟草花叶病毒（TMV）的30K蛋白被定位到受感染烟草叶片的胞间连丝中。据报道，这种蛋白存在于TMV感染的原生质体的细胞核部分，但由于有人提出它可能通过胞间连丝在病毒的细胞间运输中发挥作用，因此对完整组织进行了研究，特别关注胞间连丝和细胞核。在不同时间用TMV机械接种叶片后制作超薄切片。在孵育过程中使用针对与30K蛋白C端序列对应的合成肽的亲和纯化抗体，并将平行切片与抗TMV抗体一起孵育。接种后24小时，30K蛋白标记物在胞间连丝内积累到最大值。在细胞核或细胞的任何其他部位均未发现特异性标记。
