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人细小病毒B19 DNA超敏检测方法的开发

Development of a hypersensitive detection method for human parvovirus B19 DNA.

作者信息

Sato K, Matsuda E, Kamisango K, Iwasaki H, Matsubara S, Matsunaga Y

机构信息

Chugai Pharmaceutical Co. Ltd., Central Research Laboratories, 3-41-8 Takada, Toshima-ku, Tokyo 171-8545, Japan.

出版信息

J Clin Microbiol. 2000 Mar;38(3):1241-3. doi: 10.1128/JCM.38.3.1241-1243.2000.

DOI:10.1128/JCM.38.3.1241-1243.2000
PMID:10699031
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC86387/
Abstract

A new detection method for human parvovirus B19 DNA was established using PCR coupled with a hybridization protection assay. The amplified product was detected using acridinium ester-labeled DNA probes. By this method, a few copies of B19 DNA were detected in human serum albumin.

摘要

建立了一种将聚合酶链反应（PCR）与杂交保护分析相结合的人细小病毒B19 DNA检测新方法。使用吖啶酯标记的DNA探针检测扩增产物。通过该方法，在人血清白蛋白中检测到了少量的B19 DNA拷贝。

相似文献

1
Development of a hypersensitive detection method for human parvovirus B19 DNA.人细小病毒B19 DNA超敏检测方法的开发
J Clin Microbiol. 2000 Mar;38(3):1241-3. doi: 10.1128/JCM.38.3.1241-1243.2000.
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Tyramide signal amplification of biotinylated probe in dot-blot hybridization assay for the detection of parvovirus B19 DNA in serum samples.用于检测血清样本中B19微小病毒DNA的斑点杂交试验中生物素化探针的酪胺信号放大。
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Parvovirus B19 infection in pregnancy: quantitative viral DNA analysis using a kinetic fluorescence detection system (TaqMan PCR).妊娠期B19微小病毒感染：使用动力学荧光检测系统（TaqMan PCR）进行病毒DNA定量分析。
J Med Virol. 2002 Jun;67(2):259-66. doi: 10.1002/jmv.2216.
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J Clin Lab Anal. 2000;14(1):38-41. doi: 10.1002/(sici)1098-2825(2000)14:1<38::aid-jcla8>3.0.co;2-n.
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[Detection of parvovirus B19 in the blood of patients using DNA hybridization].
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Evaluation of different assays for the detection of parvovirus B19 DNA in human plasma.评估用于检测人血浆中B19细小病毒DNA的不同检测方法。
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Detection of parvovirus B19 by dot-blot and polymerase chain reaction.采用斑点印迹法和聚合酶链反应检测细小病毒B19
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引用本文的文献

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Quantitative competitive-PCR assay to measure human parvovirus B19-DNA load in serum samples.
Mol Biotechnol. 2006 Jan;32(1):23-9. doi: 10.1385/MB:32:1:023.
2
High-sensitivity PCR detection of parvovirus B19 in plasma.血浆中B19微小病毒的高灵敏度聚合酶链反应检测
J Clin Microbiol. 2002 Jun;40(6):1958-62. doi: 10.1128/JCM.40.6.1958-1962.2002.
3
New LightCycler PCR for rapid and sensitive quantification of parvovirus B19 DNA guides therapeutic decision-making in relapsing infections.新型LightCycler PCR技术用于快速灵敏定量检测细小病毒B19 DNA，为复发性感染的治疗决策提供指导。
J Clin Microbiol. 2001 Dec;39(12):4413-9. doi: 10.1128/JCM.39.12.4413-4419.2001.

本文引用的文献

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Screening of blood donors for human parvovirus B19 and characterization of the results.对献血者进行人细小病毒B19筛查及结果分析
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Quantitative detection of hepatitis B virus by transcription-mediated amplification and hybridization protection assay.通过转录介导扩增和杂交保护分析法定量检测乙型肝炎病毒
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Detection of all single-base mismatches in solution by chemiluminescence.通过化学发光检测溶液中的所有单碱基错配。
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Sequence analysis of a parvovirus B19 isolate and baculovirus expression of the non-structural protein.细小病毒B19分离株的序列分析及非结构蛋白的杆状病毒表达
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Sequence variability among different parvovirus B19 isolates.不同B19细小病毒分离株之间的序列变异性。
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Detection of human parvovirus B19 DNA in plasma pools and blood products derived from these pools: implications for efficiency and consistency of removal of B19 DNA during manufacture.检测血浆库及源自这些血浆库的血液制品中的人细小病毒B19 DNA：对生产过程中B19 DNA去除效率和一致性的影响。
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Large-scale screening for human parvovirus B19 DNA in clinical specimens by dot blot hybridization and polymerase chain reaction.
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Detection of human parvovirus B19 DNA by nested polymerase chain reaction.通过巢式聚合酶链反应检测人细小病毒B19 DNA
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A new genome type of human parvovirus B19 present in sera of patients with encephalopathy.一种存在于脑病患者血清中的新型人类细小病毒B19基因组类型。
J Gen Virol. 1995 Nov;76 ( Pt 11):2645-51. doi: 10.1099/0022-1317-76-11-2645.