Nutan K S, Meti B L
Department of Neurophysiology, Nimhans, Bangalore, India.
J Neurosci Res. 2000 Mar 15;59(6):806-12. doi: 10.1002/(SICI)1097-4547(20000315)59:6<806::AID-JNR13>3.0.CO;2-2.
The deficits in operant behavior and the alterations in dendritic arborizations of Cornu Ammonis 1 and Cornu Ammonis 3 (CA1 and CA3) hippocampal areas were investigated in subicular lesioned rats. The subjects were female Wistar rats aged 120 days, and were divided into four groups: one serving as age-matched untrained control, a second group received training and sham lesioning, a third group were only trained, and the fourth group were first trained and then subjected to subicular lesions. The rats were food-deprived 24 hours prior to operant behavior training sessions. Two training sessions for operant behavior with continuous reinforcement of 10 minutes duration per day were done during the shaping session, following which rats were allowed 10 minutes of operant food reward for 10 days. On the eleventh day, only the operant behavior and sham-operated rats were used for subicular lesion and sham surgery, respectively. After 72 hours of surgical recovery, operant behavioral testing was performed daily as before for a further period of 10 days. Later, all groups of rats were killed and the hippocampus was processed for rapid Golgi staining. Our results suggest that subicular lesions produce a significant reduction in operant learning. Further, the Golgi studies revealed a reduction in dendritic branching points and intersections of apical and basal CA1, CA3 neurons in lesioned rats.
在海马下托损伤的大鼠中，研究了操作性行为缺陷以及海马角1区和海马角3区（CA1和CA3）树突分支的改变。实验对象为120日龄的雌性Wistar大鼠，分为四组：一组作为年龄匹配的未训练对照组，第二组接受训练并进行假损伤，第三组仅接受训练，第四组先接受训练然后进行海马下托损伤。在操作性行为训练前24小时对大鼠进行禁食。在塑形阶段，每天进行两次持续10分钟的操作性行为训练，并给予连续强化，之后给予大鼠10分钟的操作性食物奖励，持续10天。在第11天，仅对操作性行为组和假手术组大鼠分别进行海马下托损伤和假手术。手术恢复72小时后，像之前一样每天进行操作性行为测试，持续10天。之后，处死所有组的大鼠，对海马进行快速高尔基染色处理。我们的结果表明，海马下托损伤会导致操作性学习显著减少。此外，高尔基染色研究显示，损伤大鼠的CA1、CA3神经元顶树突和基底树突的分支点和交叉点减少。
