He Y, Lee T, Leong S K
Division of Neurosurgery, Department of Surgery, National University of Singapore, Lower Kent Ridge Road, Singapore, Singapore.
Brain Res. 2000 Mar 6;858(1):163-6. doi: 10.1016/s0006-8993(99)02459-2.
The pathologic hallmark of Parkinson's disease is the dopaminergic cell death in the substantia nigra (SN). The cause of the cell death is, however, unknown. Even the question on whether the cells die by apoptosis or necrosis has not been answered with certainty. In 6-Hydroxydopamine induced Parkinsonian rats, the present study observed apoptotic nuclei from 1 day to 14 days after lesioning, using the TdT(terminal deoxynucleotidyl transferase)-mediated dUTP-biotin nick-end labeling method. Tyrosine hydroxylase immunohistochemistry and haematoxylin staining further revealed that these apoptotic cells are dopaminergic cells in the substantia nigra. The results suggest that dopaminergic cells in SN undergo apoptosis in the rat model of Parkinson's disease.
帕金森病的病理特征是黑质中多巴胺能细胞死亡。然而，细胞死亡的原因尚不清楚。甚至关于细胞是通过凋亡还是坏死死亡的问题也尚未得到确切解答。在6-羟基多巴胺诱导的帕金森病大鼠模型中，本研究采用TdT（末端脱氧核苷酸转移酶）介导的dUTP-生物素缺口末端标记法，观察了损伤后1天至14天的凋亡细胞核。酪氨酸羟化酶免疫组化和苏木精染色进一步显示，这些凋亡细胞是黑质中的多巴胺能细胞。结果表明，在帕金森病大鼠模型中，黑质中的多巴胺能细胞会发生凋亡。
