Gomez E, Diez C
Centro de Selección y Reproducción Animal, CIATA, Consejería de Agricultura, Camino de los Claveles s/n. SOMIO, 33203, Gijon Asturias, Spain.
Anim Reprod Sci. 2000 Feb 28;58(1-2):23-37. doi: 10.1016/s0378-4320(99)00078-0.
Oviductal factors may be obtained by ultrafiltration of conditioned medium, added to a simple media and used in bovine embryo culture. In this study, we aimed to analyze the development of bovine embryos produced with oviductal factors compared to those cultured in the presence of BSA or serum, the effects of glucose in presence of these protein supplements, and the ability of oviductal factors to support embryo development during the entire culture period. In vitro produced bovine zygotes from slaughterhouse ovaries were cultured in modified-synthetic oviduct fluid (mSOF) alone or supplemented with (1) oviductal factors, (2) BSA and (3) FCS. Oviductal factors showed embryotrophic activity, although with blastocyst rates lower than those in BSA and FCS. Glucose (1.5 mM) added at Day 2 of culture did not affect development in the presence of oviductal factors. The number of cells in expanded blastocysts was unaffected by the presence of glucose or any of the protein supplements used. Both BSA and FCS, respectively, improved blastocyst rates of Day 6 embryos produced with oviductal factors. The effect of oviductal factors was masked by the presence of BSA during the entire culture. FCS promoted an earlier appearance of blastocysts. It is concluded that the effect of glucose on in vitro embryo development depends upon the source of protein. Oviductal factors are not an appropriate supplement for embryos beyond Day 6 of culture in SOF, although blastocyst rates of such embryos may be increased by culturing them in the presence of FCS or BSA.
输卵管因子可通过对条件培养基进行超滤获得，添加到简单培养基中用于牛胚胎培养。在本研究中，我们旨在分析与在牛血清白蛋白（BSA）或血清存在下培养的胚胎相比，添加输卵管因子所产生的牛胚胎的发育情况、这些蛋白质补充剂存在时葡萄糖的影响以及输卵管因子在整个培养期间支持胚胎发育的能力。从屠宰场卵巢采集的体外生产的牛受精卵在单独的改良合成输卵管液（mSOF）中培养，或添加（1）输卵管因子、（2）BSA和（3）胎牛血清（FCS）进行培养。输卵管因子显示出胚胎营养活性，尽管囊胚率低于BSA和FCS组。在培养第2天添加葡萄糖（1.5 mM）在存在输卵管因子的情况下不影响发育。扩张囊胚中的细胞数量不受葡萄糖或所使用的任何蛋白质补充剂的影响。BSA和FCS分别提高了添加输卵管因子所产生的第6天胚胎的囊胚率。在整个培养过程中，BSA的存在掩盖了输卵管因子的作用。FCS促进了囊胚的更早出现。结论是，葡萄糖对体外胚胎发育的影响取决于蛋白质来源。在SOF中培养超过第6天的胚胎时，输卵管因子不是合适的补充剂，尽管在FCS或BSA存在下培养此类胚胎可能会提高囊胚率。
