羰基金属免疫分析：傅里叶变换红外光谱的一种新应用。

Carbonyl metallo immuno assay: a new application for Fourier transform infrared spectroscopy.

作者信息

Vessières A, Salmain M, Brossier P, Jaouen G

机构信息

Ecole Nationale Supérieure de Chimie de Paris, Laboratoire de Chimie Organométallique-UMR CNRS 7576, France.

出版信息

J Pharm Biomed Anal. 1999 Nov;21(3):625-33. doi: 10.1016/s0731-7085(99)00169-7.

DOI:10.1016/s0731-7085(99)00169-7

PMID:10701430

Abstract

We describe here the development of a new, non-isotopic immunological assay termed CMIA (carbonyl metallo immunoassay) that uses metal carbonyl complexes as tracers and Fourier transform infrared spectroscopy (FT-IR) as the detection method. This assay is based on the particular spectral features of these complexes, which show very strong absorption bands in the 1,800-2,200 cm(-1) spectral range where proteins and organic molecules do not absorb. In Section 1, the optimisation of the quantitative detection of these tracers is detailed. In Section 2, the implementation of mono-CMIA is described, including the CMIA assays of three antiepileptic drugs (carbamazepine, phenobarbital, phenytoin). Finally, extension to the simultaneous double- and triple-CMIA of these drugs is reported.

摘要

我们在此描述一种名为CMIA（羰基金属免疫分析）的新型非同位素免疫分析方法的开发，该方法使用金属羰基配合物作为示踪剂，并采用傅里叶变换红外光谱（FT-IR）作为检测方法。该分析基于这些配合物的特定光谱特征，它们在1800 - 2200 cm⁻¹光谱范围内显示出非常强的吸收带，而蛋白质和有机分子在此范围内不吸收。在第1节中，详细介绍了这些示踪剂定量检测的优化。在第2节中，描述了单CMIA的实施，包括三种抗癫痫药物（卡马西平、苯巴比妥、苯妥英）的CMIA分析。最后，报告了将这些药物扩展到同时进行双CMIA和三CMIA分析的情况。