人类软骨相关蛋白（CRTAP）编码基因的cDNA克隆、特性分析及染色体定位

cDNA cloning, characterization and chromosome mapping of the gene encoding human cartilage associated protein (CRTAP).

作者信息

Tonachini L, Morello R, Monticone M, Skaug J, Scherer S W, Cancedda R, Castagnola P

机构信息

Istituto Nazionale per la Ricerca sul Cancro, Centro di Biotecnologie Avanzate, Genova, Italy.

出版信息

Cytogenet Cell Genet. 1999;87(3-4):191-4. doi: 10.1159/000015463.

DOI:10.1159/000015463

PMID:10702664

Abstract

We have recently isolated and characterized cDNA clones coding for a novel developmentally regulated avian and mouse embryo protein, CASP for Cartilage Associated Protein. Here we describe the isolation and characterization of the gene coding for the human CASP. The comparison of the putative human and mouse protein sequences with the chick sequence revealed an overall high identity (89% and 51%, respectively). Homology search with known DNA and protein sequences showed that CASPs are related to two mammalian nuclear proteins. Here we demonstrate definitively that CASPs are distinct from these nuclear proteins. However, sequence comparison analyses suggest that all of these proteins belong to a new family. In all human tissues examined two CASP mRNA species were detected, whereas a single mRNA and three mRNAs were found in chick and mouse, respectively. The human CASP gene (CRTAP) was assigned to chromosome 3p22 by fluorescence in situ hybridization.

摘要

我们最近分离并鉴定了编码一种新型发育调控的禽类和小鼠胚胎蛋白（软骨相关蛋白，简称CASP）的cDNA克隆。在此，我们描述了人类CASP编码基因的分离和鉴定。将推测的人类和小鼠蛋白质序列与鸡的序列进行比较，发现总体上具有高度同源性（分别为89%和51%）。与已知DNA和蛋白质序列进行同源性搜索表明，CASP与两种哺乳动物核蛋白相关。在此我们明确证明，CASP与这些核蛋白不同。然而，序列比较分析表明，所有这些蛋白质都属于一个新家族。在所检测的所有人类组织中，检测到两种CASP mRNA种类，而在鸡和小鼠中分别发现一种mRNA和三种mRNA。通过荧光原位杂交，将人类CASP基因（CRTAP）定位于3号染色体p22区域。