[博德特氏菌转座子元件在大肠杆菌K12中的转位与遗传]
[Transposition and inheritance of Bordetella Tn-element in Escherichia coli K12].
作者信息
Nechaeva E V, Sivov I G, Karataev G I
出版信息
Mol Gen Mikrobiol Virusol. 2000(1):20-3.
PMID:10702986
Abstract
B. pertussis genetically mobile element TnBp3 integrates the plasmid in E. coli chromosome. During culturing under nonselective conditions the majority of cells of some E. coli strains lose the kanamycin resistance marker, which indicates the instability of TnBp3 inheriting. The stability of inheriting the integrated structure is higher in E. coli cells with recB-21 recC-12 sbcB-2 mutations. The role of RecBC recombination system in extrusion of TnBp3 is discussed.
摘要
百日咳博德特氏菌的遗传可移动元件TnBp3将质粒整合到大肠杆菌染色体中。在非选择性条件下培养时，一些大肠杆菌菌株的大多数细胞会失去卡那霉素抗性标记，这表明TnBp3遗传的不稳定性。在具有recB-21 recC-12 sbcB-2突变的大肠杆菌细胞中，整合结构遗传的稳定性更高。文中讨论了RecBC重组系统在TnBp3挤出中的作用。
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