甘露聚糖对贯叶连翘茎段培养物中金丝桃素生成的刺激作用。
Stimulation of the production of hypericins by mannan in Hypericum perforatum shoot cultures.
作者信息
Kirakosyan A, Hayashi H, Inoue K, Charchoglyan A, Vardapetyan H
机构信息
Department of Pharmacognosy, Gifu Pharmaceutical University, Japan.
出版信息
Phytochemistry. 2000 Feb;53(3):345-8. doi: 10.1016/s0031-9422(99)00496-3.
Shoot organ cultures were established from callus derived from anthers of Hypericum perforatum flowers and the effect of elicitors on hypericin and pseudohypericin production in shoot organ cultures was investigated. Mannan stimulated pseudohypericin production up to four fold (0.82 mg/g dry wt) and hypericin production up to two fold (0.04 mg/g dry wt.) beta-1,3-glucan and pectin slightly stimulated pseudohypericin production (ca. two fold), but had no effect on hypericin production. On the other hand, yeast extract showed no stimulatory effect, on either hypericin or pseudohypericin production.
从贯叶连翘花的花药愈伤组织中建立了茎尖器官培养物，并研究了诱导子对茎尖器官培养物中金丝桃素和假金丝桃素产生的影响。甘露聚糖使假金丝桃素产量提高了四倍（0.82毫克/克干重），使金丝桃素产量提高了两倍（0.04毫克/克干重）。β-1,3-葡聚糖和果胶略微刺激了假金丝桃素的产生（约两倍），但对金丝桃素的产生没有影响。另一方面，酵母提取物对金丝桃素或假金丝桃素的产生均无刺激作用。
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