Department of Integrative Plant Biology, Institute of Plant Genetics of the Polish Academy of Sciences, Poznań, Wielkopolska, Poland.
Chinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi, China.
J Pharm Pharmacol. 2019 Jan;71(1):70-82. doi: 10.1111/jphp.12743. Epub 2017 May 19.
In this review, we aim at updating the available information on the improvement of the Hypericum perforatum L. (Hypericaceae) phytochemical profile and pharmacological properties via elicitation.
Hypericum perforatum seedlings, shoots, roots, calli and cell suspension cultures were treated with diverse elicitors to induce the formation of secondary metabolites. The extracts of the elicitor-treated plant material containing naphthodianthrones, phloroglucinols, xanthones, flavonoids and other new compounds were quantitatively analysed and tested for their bioactivities. While hypericins were mainly produced in H. perforatum cultures containing dark nodules, namely shoots and seedlings, other classes of compounds such as xanthones, phloroglucinols and flavonoids were formed in all types of cultures. The extracts obtained from elicitor-treated samples generally possessed better bioactivities compared to the extract of control biomass.
Although elicitation is an excellent tool for the production of valuable secondary metabolites in H. perforatum cell and tissue cultures, its exploitation is still in its infancy mainly due to the lack of reproducibility and difficulties in scaling up biomass production.
在本次综述中，我们旨在更新有关通过诱导提高贯叶连翘（藤黄科）植物化学成分和药理特性的现有信息。
用多种诱导剂处理贯叶连翘幼苗、茎、根、愈伤组织和细胞悬浮培养物，以诱导次生代谢产物的形成。对含萘并二蒽酮、苯丙素醇、呫吨酮、黄酮类化合物和其他新化合物的诱导剂处理植物材料提取物进行定量分析，并测试其生物活性。虽然金丝桃素主要在含有暗结节的贯叶连翘培养物（即茎和幼苗）中产生，而其他类化合物如呫吨酮、苯丙素醇和黄酮类化合物则在所有类型的培养物中形成。与对照生物量的提取物相比，从诱导剂处理样品中获得的提取物通常具有更好的生物活性。
尽管诱导是在贯叶连翘细胞和组织培养物中生产有价值的次生代谢产物的绝佳工具，但由于缺乏重现性和扩大生物量生产的困难，其应用仍处于起步阶段。
