Roth C, Schuierer M, Günther K, Buettner R
Institute for Pathology, University of Regensburg Medical School, Franz-Josef-Strauss-Allee, Regensburg, D-93042, Germany.
Genomics. 2000 Feb 1;63(3):384-90. doi: 10.1006/geno.1999.6084.
We recently cloned a novel murine transcriptional repressor, the Krüppel-like zinc finger protein AP-2rep (HGMW-approved symbol KLF12), that binds to a regulatory element in the AP-2alpha gene promoter. In the present study, we characterize the human AP-2rep homolog and describe expression patterns in human urogenital and lymphoma cell lines. The predicted human protein of 402 amino acids exhibits 95.8% identity and 98.5% similarity to the murine AP-2rep peptide. The genomic locus of human AP-2rep consists of seven exons and was assigned to chromosome 13q22 by fluorescence in situ hybridization to metaphase chromosomes. Human AP-2rep repressed both reporter expression from a transiently transfected AP-2alpha promoter and the endogenous AP-2alpha gene and inversely was negatively regulated by AP-2alpha. The consensus motif CAGTGGG was identified by an in vitro binding site selection assay. In summary, our data further point to an important role of AP-2rep as a transcriptional silencer and reveal reciprocal regulation of AP-2alpha and AP-2rep.
我们最近克隆了一种新型小鼠转录抑制因子，即Krüppel样锌指蛋白AP-2rep（HGMW批准符号为KLF12），它可与AP-2α基因启动子中的调控元件结合。在本研究中，我们对人AP-2rep同源物进行了表征，并描述了其在人泌尿生殖系和淋巴瘤细胞系中的表达模式。预测的含402个氨基酸的人蛋白与小鼠AP-2rep肽的同一性为95.8%，相似性为98.5%。人AP-2rep的基因组位点由7个外显子组成，并通过对中期染色体的荧光原位杂交定位到染色体1q22。人AP-2rep既抑制瞬时转染的AP-2α启动子的报告基因表达，也抑制内源性AP-2α基因，反之，它受到AP-2α的负调控。通过体外结合位点筛选试验鉴定出共有基序CAGTGGG。总之，我们的数据进一步表明AP-2rep作为转录沉默因子的重要作用，并揭示了AP-2α与AP-2rep的相互调控。
