Pauly J L
J Med. 1976;7(3-4):283-95.
Cultured human lymphoblastoid cells of different lines elaborate rapidly pyrimidine-specific nucleosides when incubated as a dense population in a serum-containing milieu. No appreciable changes in viability or morphology were detected in the time required for maximal enzyme production. These nucleosidases degraded thymidine, uridine and cytidine; adenosine and guanosine were not catabolized. Cell viability and proliferation were not affected by these heat-labile and ultraviolet-sensitive enzymes. Products arising from nucleosidase degradation of radiolabeled pyrimidines, which were separated and identified by paper chromatography, were not incorporated by DNA- and RNA-synthesizing lymphoid or nonlymphoid cells.
当在含血清的环境中以高密度群体培养时，不同细胞系的人培养淋巴母细胞会迅速合成嘧啶特异性核苷。在产生最大量酶所需的时间内，未检测到活力或形态有明显变化。这些核苷酶可降解胸腺嘧啶核苷、尿苷和胞苷；腺苷和鸟苷不被分解代谢。这些热不稳定且对紫外线敏感的酶不会影响细胞活力和增殖。通过纸层析分离和鉴定的放射性标记嘧啶核苷酶降解产物，未被合成DNA和RNA的淋巴细胞或非淋巴细胞摄取。
