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精氨酸支原体感染的细胞因核苷的酶促裂解而抑制[3H]胸苷掺入。

Inhibition of [3H]thymidine incorporation by Mycoplasma arginini-infected cells due to enzymatic cleavage of the nucleoside.

作者信息

Sinigaglia F, Talmadge K W

出版信息

Eur J Immunol. 1985 Jul;15(7):692-6. doi: 10.1002/eji.1830150710.

DOI:10.1002/eji.1830150710
PMID:3874081
Abstract

Culture supernatants contaminated by Mycoplasma arginini inhibit the incorporation of [3H]thymidine ([3H]dThd) by cytotoxic T lymphocyte cell lines. This study presents evidence that the inhibition of uptake of the nucleoside is due to the rapid cleavage of the exogenous [3H]dThd into thymine. Uridine and cytidine as well as dThd are degraded by the mycoplasma-contaminated supernatants, while no cleavage was observed with uninfected supernatants. Cells contaminated by mycoplasma apparently release a pyrimidine-specific nucleosidase, possibly a dThd phosphorylase, which is responsible for the inhibition.

摘要

被精氨酸支原体污染的培养上清液会抑制细胞毒性T淋巴细胞系对[3H]胸腺嘧啶核苷（[3H]dThd）的掺入。本研究表明，核苷摄取受到抑制是由于外源性[3H]dThd迅速裂解为胸腺嘧啶。被支原体污染的上清液会降解尿苷、胞苷以及dThd，而未感染的上清液则未观察到裂解现象。被支原体污染的细胞显然会释放一种嘧啶特异性核苷酶，可能是一种dThd磷酸化酶，这就是造成抑制的原因。

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Inhibition of [3H]thymidine incorporation by Mycoplasma arginini-infected cells due to enzymatic cleavage of the nucleoside.精氨酸支原体感染的细胞因核苷的酶促裂解而抑制[3H]胸苷掺入。
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Short fluorodeoxyuridine exposure of different human glioblastoma lines induces high-level accumulation of S-phase cells that avidly incorporate 125I-iododeoxyuridine.不同人类胶质母细胞瘤细胞系短期暴露于氟脱氧尿苷会诱导S期细胞高水平积累，这些细胞会大量掺入125I-碘脱氧尿苷。
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