Ghosh S, Klobutcher L A
Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.
Genesis. 2000 Mar;26(3):179-88. doi: 10.1002/(sici)1526-968x(200003)26:3<179::aid-gene3>3.0.co;2-b.
During the process of macronuclear development, the ciliate Euplotes crassus undergoes extensive programmed DNA rearrangement. Previous studies have identified a gene, H3(P), that is expressed only during sexual reproduction and is predicted to encode a variant histone H3 protein. In the current study, an antiserum to the H3(P) protein has been generated. The antiserum has been used to demonstrate that H3(P) is maximally expressed during the polytene chromosome stage of macronuclear development. Moreover, H3(P) is localized to the developing macronucleus, but not other nuclei present within the cell. Additional studies indicate that at least one additional variant histone is also present within the developing macronucleus. The results indicate that there are significant changes in nucleosome composition within the developing macronucleus, and provide additional support for the notion that changes in chromatin structure play a role in the DNA rearrangement processes of macronuclear development. genesis 26:179-188, 2000.
在大核发育过程中，纤毛虫厚尾真核草履虫经历广泛的程序性DNA重排。先前的研究鉴定出一个基因H3(P)，它仅在有性生殖期间表达，预计编码一种变体组蛋白H3蛋白。在当前研究中，已制备出针对H3(P)蛋白的抗血清。该抗血清已用于证明H3(P)在大核发育的多线染色体阶段表达量最高。此外，H3(P)定位于发育中的大核，但不定位于细胞内的其他细胞核。进一步的研究表明，发育中的大核内还存在至少一种其他变体组蛋白。结果表明，发育中的大核内核小体组成有显著变化，并为染色质结构变化在大核发育的DNA重排过程中起作用这一观点提供了更多支持。《基因》2000年第26卷：179 - 188页
