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Development of a plasmid vector for easy selection of strong promoters.

作者信息

Piñeiro S A, Sordelli D O, Centrón D

机构信息

Laboratorio BioSidus S.A., (1254) Constitución 4234, Buenos Aires, Argentina.

出版信息

Curr Microbiol. 2000 May;40(5):302-5. doi: 10.1007/s002849910060.

DOI:10.1007/s002849910060
PMID:10706659
Abstract

A promoter vector pACPR33 for Escherichia coli based on the promotorless ampicillin-resistance gene from pBR322 has been constructed. The promoter of the ampicillin-resistance gene was deleted and replaced by a suitable multiple cloning site. Molecular cloning of promoters into the polylinker resulted in activation of the ampicillin resistance in E. coli. The plasmid contains a functional origin of DNA replication and a tetracycline resistance gene for E. coli, and a chloramphenicol resistance gene for S. aureus. The vector permitted direct detection of promoter activity, especially strong promoters, by easy iodometric determination of beta-lactamase activity in liquid or solid media.

摘要

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