Li T, Sun F, Yuan Z, Zhang Y, Yu J, Pang Y
Wuhan Institute of Virology, Academia Sinica, Wuhan 430071, China.
Curr Microbiol. 2000 May;40(5):322-6. doi: 10.1007/s002849910064.
The cyt1Aa gene of Bacillus thuringiensis subsp. israelensis and binary toxin gene of Bacillus sphaericus C3-41 were introduced into an acrystalliferous strain of B. thuringiensis independently and in combination by using shuttle vector pBU4. SDS-PAGE and Western blot analysis proved that cyt1Aa and binary toxin genes coexpressed during the sporulation of the recombinant. Transformant strain expressing the Cyt1Aa and binary toxin proteins in combination was more toxic to susceptible and resistant Culex pipiens quinquefasciatus than the transformants expressing Cyt1Aa protein or binary toxin proteins independently. It was suggested that large amount of production of Cyt1Aa protein and binary toxin proteins possibly interacted synergistically, thereby increasing its mosquitocidal toxicity significantly.
通过穿梭载体pBU4，将苏云金芽孢杆菌以色列亚种的cyt1Aa基因和球形芽孢杆菌C3 - 41的二元毒素基因分别单独以及组合导入无晶体的苏云金芽孢杆菌菌株中。SDS - PAGE和Western blot分析证明，cyt1Aa和二元毒素基因在重组体芽孢形成过程中共同表达。与单独表达Cyt1Aa蛋白或二元毒素蛋白的转化体相比，组合表达Cyt1Aa和二元毒素蛋白的转化菌株对敏感和抗性致倦库蚊毒性更强。这表明大量产生的Cyt1Aa蛋白和二元毒素蛋白可能发生协同作用，从而显著提高其杀蚊毒性。
