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产生Cyt1A、Cry11B和球形芽孢杆菌二元毒素的苏云金芽孢杆菌重组菌株。

Recombinant strain of Bacillus thuringiensis producing Cyt1A, Cry11B, and the Bacillus sphaericus binary toxin.

作者信息

Park Hyun-Woo, Bideshi Dennis K, Federici Brian A

机构信息

Department of Entomology, University of California, Riverside, California 92521, USA.

出版信息

Appl Environ Microbiol. 2003 Feb;69(2):1331-4. doi: 10.1128/AEM.69.2.1331-1334.2003.

DOI:10.1128/AEM.69.2.1331-1334.2003
PMID:12571069
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC143673/
Abstract

A novel recombinant Bacillus thuringiensis subsp. israelensis strain that produces the B. sphaericus binary toxin, Cyt1Aa, and Cry11Ba is described. The toxicity of this strain (50% lethal concentration [LC(50)] = 1.7 ng/ml) against fourth-instar Culex quinquefasciatus was higher than that of B. thuringiensis subsp. israelensis IPS-82 (LC(50) = 7.9 ng/ml) or B. sphaericus 2362 (LC(50) = 12.6 ng/ml).

摘要

描述了一种新型重组苏云金芽孢杆菌以色列亚种菌株，该菌株可产生球形芽孢杆菌二元毒素、Cyt1Aa和Cry11Ba。该菌株对致倦库蚊四龄幼虫的毒性（半数致死浓度[LC(50)] = 1.7 ng/ml）高于苏云金芽孢杆菌以色列亚种IPS-82（LC(50) = 7.9 ng/ml）或球形芽孢杆菌2362（LC(50) = 12.6 ng/ml）。

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Recombinant strain of Bacillus thuringiensis producing Cyt1A, Cry11B, and the Bacillus sphaericus binary toxin.产生Cyt1A、Cry11B和球形芽孢杆菌二元毒素的苏云金芽孢杆菌重组菌株。
Appl Environ Microbiol. 2003 Feb;69(2):1331-4. doi: 10.1128/AEM.69.2.1331-1334.2003.
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本文引用的文献

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Influence of Exposure to Single versus Multiple Toxins of Bacillus thuringiensis subsp. israelensis on Development of Resistance in the Mosquito Culex quinquefasciatus (Diptera: Culicidae).苏云金芽孢杆菌亚种 israelensis 单一毒素与多种毒素暴露对致倦库蚊发育抗性的影响（双翅目：蚊科）。
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Cyt1A from Bacillus thuringiensis restores toxicity of Bacillus sphaericus against resistant Culex quinquefasciatus (Diptera: Culicidae).来自苏云金芽孢杆菌的Cyt1A可恢复球形芽孢杆菌对抗性致倦库蚊（双翅目：蚊科）的毒性。
J Med Entomol. 2000 May;37(3):401-7.
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Construction and characterization of a recombinant Bacillus thuringiensis subsp. israelensis strain that produces Cry11B.产生Cry11B的苏云金芽孢杆菌以色列亚种重组菌株的构建与特性分析
J Invertebr Pathol. 2001 Jul;78(1):37-44. doi: 10.1006/jipa.2001.5038.
4
Domain I plays an important role in the crystallization of Cry3A in Bacillus thuringiensis.结构域I在苏云金芽孢杆菌中Cry3A的结晶过程中发挥着重要作用。
Mol Biotechnol. 2000 Oct;16(2):97-107. doi: 10.1385/MB:16:2:97.
5
Differential enhancement of Cry2A versus Cry11A yields in Bacillus thuringiensis by use of the cry3A STAB mRNA sequence.通过使用cry3A STAB mRNA序列提高苏云金芽孢杆菌中Cry2A与Cry11A产量的差异增强。
FEMS Microbiol Lett. 1999 Dec 15;181(2):319-27. doi: 10.1111/j.1574-6968.1999.tb08862.x.
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Potency of products based on Bacillus thuringiensis var. israelensis: interlaboratory variations.
J Am Mosq Control Assoc. 1998 Sep;14(3):298-304.
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Optimization of Cry3A yields in Bacillus thuringiensis by use of sporulation-dependent promoters in combination with the STAB-SD mRNA sequence.通过使用与STAB-SD mRNA序列结合的芽孢形成依赖性启动子来优化苏云金芽孢杆菌中Cry3A的产量。
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Bacillus thuringiensis and its pesticidal crystal proteins.苏云金芽孢杆菌及其杀虫晶体蛋白。
Microbiol Mol Biol Rev. 1998 Sep;62(3):775-806. doi: 10.1128/MMBR.62.3.775-806.1998.
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Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein.来自苏云金芽孢杆菌jegathesan亚种的一个编码高杀蚊蛋白的新型毒素基因的克隆与表达。
Appl Environ Microbiol. 1995 Dec;61(12):4230-5. doi: 10.1128/aem.61.12.4230-4235.1995.
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J Bacteriol. 1993 Aug;175(16):5276-80. doi: 10.1128/jb.175.16.5276-5280.1993.