Deeb S S, Diller L C, Williams D R, Dacey D M
Department of Medicine, University of Washington, Seattle 98195, USA.
J Opt Soc Am A Opt Image Sci Vis. 2000 Mar;17(3):538-44. doi: 10.1364/josaa.17.000538.
We analyzed the ratio of L:M cone photopigment mRNA in the retinas of Old World monkeys, using the method of rapid polymerase chain reaction-single-strand conformation polymorphism. The L:M cone pigment mRNA ratio in whole retina ranged from 0.6 to 7.0, with a mean of approximately 1.6 (standard deviation, +/- 0.56; n = 26). There was no change in this ratio with eccentricity up to 9 mm (approximately 45 degrees), though the ratio was approximately 30% greater in temporal than in nasal retina. The mRNA ratios are in good agreement with the L:M cone ratio in these same retinas, inferred from electrophysiological recordings of cone signal gain in horizontal cell interneurons. The correlation between mRNA ratios and physiological cone gain ratio supports the conclusion that both measures reflect the relative number of L and M cones.
我们采用快速聚合酶链反应-单链构象多态性方法，分析了旧世界猴视网膜中L:M视锥色素mRNA的比例。整个视网膜中L:M视锥色素mRNA比例在0.6至7.0之间，平均约为1.6（标准差，±0.56；n = 26）。在离心率达9毫米（约45度）范围内，该比例没有变化，不过颞侧视网膜中的比例比鼻侧视网膜大约高30%。这些mRNA比例与通过水平细胞中间神经元中视锥信号增益的电生理记录推断出的相同视网膜中的L:M视锥比例高度一致。mRNA比例与生理视锥增益比例之间的相关性支持了这一结论：这两种测量方法均反映了L视锥和M视锥的相对数量。
