Recio J A, Zambrano N, Peña L d, Reig J A, Rhoads A, Rouzaut A, Notario V
Laboratory of Experimental Carcinogenesis, Department of Radiation Medicine, Georgetown University Medical Center, Washington, D.C 20007, USA.
Mol Carcinog. 2000 Mar;27(3):229-36.
We identified a human cDNA encoding a 47-kDa protein that shares 78% and 87% identity with the products of the Syrian hamster and mouse PCPH proto-oncogenes respectively. The human homolog was localized by radiation-hybrid mapping to chromosome band 14q24.3, a region syntenic to the Pcph location on mouse chromosome 12. Northern analyses revealed that PCPH mRNA was widely distributed in normal human adult tissues, but its expression varied significantly among human tumor cells and cell lines of several tissue types, regardless of the level of expression in the corresponding normal tissues. The highest levels of PCPH mRNA and protein were detected in kidney and liver. However, PCPH was not expressed in the majority of human neoplasms tested, including kidney tumors. These data provide suggestive evidence for a possible association of the lack of PCPH expression to the neoplastic phenotype of human tumor cells. Our results should prove instrumental in designing studies to define the cellular function of the human PCPH proto-oncogene.
我们鉴定出一种编码47 kDa蛋白质的人类cDNA，它与叙利亚仓鼠和小鼠PCPH原癌基因的产物分别具有78%和87%的同一性。通过辐射杂种图谱将人类同源物定位到染色体带14q24.3，该区域与小鼠染色体12上的Pcph位置同线。Northern分析显示，PCPH mRNA在正常成人组织中广泛分布，但其在几种组织类型的人类肿瘤细胞和细胞系中的表达差异显著，与相应正常组织中的表达水平无关。在肾脏和肝脏中检测到最高水平的PCPH mRNA和蛋白质。然而，在大多数测试的人类肿瘤中，包括肾肿瘤，PCPH不表达。这些数据为PCPH表达缺失与人类肿瘤细胞的肿瘤表型之间可能存在关联提供了暗示性证据。我们的结果应有助于设计研究来确定人类PCPH原癌基因的细胞功能。
