Huber M A, Kraut N, Addicks T, Peter R U
Department of Dermatology, University of Ulm, Oberer Eselsberg 40 (BWK), Ulm, 89081, Germany.
Biochem Biophys Res Commun. 2000 Mar 16;269(2):546-52. doi: 10.1006/bbrc.2000.2287.
Eotaxin is an eosinophil-specific C-C chemokine that is implicated in the pathogenesis of eosinophilic inflammatory diseases, such as asthma and atopic dermatitis, by acting specifically on its receptor CCR3. Using RT-PCR analysis, we show that the expression of eotaxin is upregulated upon treatment with ionizing radiation (IR) in human dermal fibroblasts, but not in the bronchial epithelial cell line A549. In contrast, the gene encoding CCR3 is markedly induced in both cell types. None of the genes coding for other CCR3 ligands are significantly induced by IR in these cell types. cDNA array analysis of irradiated versus nonirradiated A549 cells and human dermal fibroblasts confirm and extend these results, and support the observation that regulation of eotaxin/CCR3-induction by IR occurs in a selective and cell-type-dependent manner. They further suggest that the induction of signaling via eotaxin and CCR3 may be an important step leading to eosinophilia in patients with radiation exposure.
嗜酸性粒细胞趋化因子是一种嗜酸性粒细胞特异性的C-C趋化因子，通过特异性作用于其受体CCR3，参与嗜酸性粒细胞炎症性疾病（如哮喘和特应性皮炎）的发病机制。通过逆转录聚合酶链反应（RT-PCR）分析，我们发现，电离辐射（IR）处理后人皮肤成纤维细胞中嗜酸性粒细胞趋化因子的表达上调，但支气管上皮细胞系A549中则不然。相反，编码CCR3的基因在这两种细胞类型中均显著诱导表达。在这些细胞类型中，编码其他CCR3配体的基因均未被IR显著诱导。对辐照和未辐照的A549细胞及人皮肤成纤维细胞进行的cDNA阵列分析证实并扩展了这些结果，并支持以下观察结果：IR对嗜酸性粒细胞趋化因子/CCR3诱导的调节以选择性和细胞类型依赖性方式发生。它们进一步表明，通过嗜酸性粒细胞趋化因子和CCR3诱导信号传导可能是辐射暴露患者发生嗜酸性粒细胞增多症的重要步骤。
