Gremiakova T A, Volkovoĭ K I, Shaĭkhutdinova R Z, Stepanov A V
Vestn Ross Akad Med Nauk. 1999(12):32-4.
The preparations of pilopolysaccharides (LPS) of virulent and avirulent Y. pestis strains, which have a different composition, were cultivated at different temperatures. Despite the cultivation temperature of parental cells, all LPS preparation inhibited the passive hemagglutination reaction (PHR) of the red blood cells sensitized by LPS isolated from the cultures grown at 26 degrees C by using homologous antisera. In contrast, the homologous system consisting of the red blood cells sensitized by LPS from the EV NIIEG, cultured at 37 degrees C, and the antiserum to these cells proved to be more specific and it was inhibited only by the homologous LPS isolated from the strain EV NIIEG. The similar reaction of the interaction of the red blood cells sensitized by high temperature LPS agents from plasmid-free strains with the same serum was inhibited by all plague LPS preparations. The LPS preparations from the strains Y. pestis 1146 and Y. pseudotuberculosis 9532 obtained when cultivated at 37 degrees C. RHR of the red blood cells sensitized by these preparations was inhibited by homologous LPS irrespective of the temperature of cell cultivation. In all cases, the reaction was specific for Yersinia strains and it was inhibited by LPS from Ra-Rd2 cultures, variants of Salmonella and E. coli.
具有不同组成的强毒和无毒鼠疫耶尔森氏菌菌株的脂多糖(LPS)制剂在不同温度下培养。尽管亲本细胞的培养温度不同,但所有LPS制剂均抑制了使用同源抗血清对在26℃下培养的培养物中分离出的LPS致敏的红细胞的被动血凝反应(PHR)。相反,由在37℃下培养的EV NIIEG的LPS致敏的红细胞和针对这些细胞的抗血清组成的同源系统被证明更具特异性,并且仅被从菌株EV NIIEG分离出的同源LPS抑制。所有鼠疫LPS制剂均抑制了无质粒菌株的高温LPS制剂致敏的红细胞与同一种血清相互作用的类似反应。在37℃下培养时获得的鼠疫耶尔森氏菌1146菌株和假结核耶尔森氏菌9532菌株的LPS制剂。这些制剂致敏的红细胞的RHR被同源LPS抑制,而与细胞培养温度无关。在所有情况下,该反应对耶尔森氏菌菌株具有特异性,并且被来自沙门氏菌和大肠杆菌变体Ra-Rd2培养物的LPS抑制。