通过改良的铁摄取调节蛋白滴定法（FURTA-Hp）鉴定幽门螺杆菌的铁调节基因。
Identification of iron-regulated genes of Helicobacter pylori by a modified fur titration assay (FURTA-Hp).
作者信息
Fassbinder F, van Vliet A H, Gimmel V, Kusters J G, Kist M, Bereswill S
机构信息
University of Freiburg, Institute of Medical Microbiology and Hygiene, Department of Microbiology, Hermann-Herder-Str. 11, D-79104, Freiburg, Germany.
出版信息
FEMS Microbiol Lett. 2000 Mar 15;184(2):225-9. doi: 10.1111/j.1574-6968.2000.tb09018.x.
The Escherichia coli-based Fur titration assay (FURTA), although a powerful tool for identification of genes regulated by the ferric uptake regulator (Fur), was unsuccessful for the gastric pathogen Helicobacter pylori. The FURTA was modified by construction of an E. coli indicator strain producing H. pylori Fur only. The promoter regions of the ferric citrate receptor homolog fecA2 and the riboflavin synthesis gene ribBA were both positive in the modified FURTA, but negative in the original FURTA. Transcription of fecA2 and ribBA was demonstrated to be iron-repressed in H. pylori. This type of modification should allow FURTA analysis for bacteria with Fur binding sequences poorly recognized by E. coli Fur.
基于大肠杆菌的铁摄取调节蛋白（Fur）滴定分析（FURTA），虽然是鉴定受铁摄取调节蛋白（Fur）调控基因的有力工具，但对胃病原体幽门螺杆菌却未成功。通过构建仅产生幽门螺杆菌Fur的大肠杆菌指示菌株对FURTA进行了改良。柠檬酸铁受体同源物fecA2和核黄素合成基因ribBA的启动子区域在改良的FURTA中均为阳性，但在原始FURTA中为阴性。已证明fecA2和ribBA的转录在幽门螺杆菌中受铁抑制。这种类型的改良应能使FURTA分析适用于具有大肠杆菌Fur难以识别的Fur结合序列的细菌。
Zotero 插件