Bereswill S, Lichte F, Vey T, Fassbinder F, Kist M
Institute of Medical Microbiology and Hygiene, Department of Microbiology and Hygiene, Freiburg, Germany.
FEMS Microbiol Lett. 1998 Feb 15;159(2):193-200. doi: 10.1111/j.1574-6968.1998.tb12860.x.
The fur homologue of Helicobacter pylori was isolated by screening a plasmid-based, genomic DNA library using the Fur titration assay (FURTA). The analysis of the DNA sequence revealed significant homology with Fur proteins from various other bacterial species. The highest degree of homology was observed for the Fur protein from Campylobacter jejuni. The H. pylori fur gene on a plasmid could partially complement the fur mutation in Escherichia coli strain H1681. The repressor activity depended on addition of iron to the medium indicating that iron acts as a co-repressor for the H. pylori protein similar to Fur from other bacteria. Comparison of Fur from H. pylori strain NCTC11638 with the recently published genomic DNA sequence of another strain (26695) confirmed the identity of the fur homologue and revealed that the fur locus is highly conserved in both strains.
通过使用铁摄取调节蛋白滴定分析（FURTA）筛选基于质粒的基因组DNA文库，分离出幽门螺杆菌的铁摄取调节蛋白同源物。DNA序列分析显示，它与来自各种其他细菌物种的铁摄取调节蛋白具有显著同源性。观察到与空肠弯曲杆菌的铁摄取调节蛋白同源性最高。质粒上的幽门螺杆菌铁摄取调节蛋白基因可以部分弥补大肠杆菌H1681菌株中的铁摄取调节蛋白突变。阻遏物活性取决于向培养基中添加铁，这表明铁作为幽门螺杆菌蛋白的共阻遏物，类似于其他细菌的铁摄取调节蛋白。将幽门螺杆菌NCTC11638菌株的铁摄取调节蛋白与另一菌株（26695）最近公布的基因组DNA序列进行比较，证实了铁摄取调节蛋白同源物的一致性，并表明铁摄取调节蛋白基因座在这两种菌株中高度保守。
