Hayashi H, Sato Y, Kanai S, Masuda M, Ohta M, Funakoshi A, Nagao K, Imaizumi K, Miyasaka K
Department of Internal Medicine, Yokohama Red Cross Hospital, Japan.
Mech Ageing Dev. 2000 Feb 15;113(3):219-25. doi: 10.1016/s0047-6374(99)00110-4.
Lymphatic lipid transport in the intestine of adult and ageing rats was compared. Adult (8-10 months old) and old (24-26 months old) male Wistar rats were cannulated into the mesenteric lymph under ethrane anesthesia. On the following day, lipid emulsion containing 35.4 mg/h of olive oil was infused intraduodenally for 7 h and lymph collected hourly was assayed for triglyceride and apolipoprotein A-IV (apo A-IV). The results showed there was no difference in lymphatic lipid and apo A-IV transport between adult and old rats. Since apo A-IV synthesis in the enterocytes is linked to the intracellular assembly of lipoprotein, it is likely that in addition to lymphatic transport, production of chylomicrons is not impaired in ageing rats.
