Prabhu S S, Broaddus W C, Oveissi C, Berr S S, Gillies G T
Division of Neurosurgery, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298, USA.
IEEE Trans Biomed Eng. 2000 Feb;47(2):259-65. doi: 10.1109/10.821776.
The measurement of tumor volumes is a practical and objective method of assessing the efficacy of a therapeutic agent. However, the relative accuracy of different methods of assessing tumor volume has been unclear. Using T1-weighted, gadolinium-enhanced magnetic resonance Imaging (T1-MRI), Evans Blue infusion and histology we measured intracranial tumor volumes in a rodent brain tumor model (RT2) at days 10, 16 and 18 after implantation of cells in the caudate putamen. There is a good correlation between tumor volumes comparing T1-MRI and Evans Blue (r2 = 0.99), T1-MRI and Histology (r2 = 0.98) and histology and Evans Blue (r2 = 0.93). Each of these methods is reliable in estimating tumor volumes in laboratory animals. There was significant uptake of gadolinium and Evans Blue in the tumor suggesting a wide disruption of the blood-brain barrier.
测量肿瘤体积是评估治疗药物疗效的一种实用且客观的方法。然而，不同的肿瘤体积评估方法的相对准确性尚不清楚。我们利用T1加权、钆增强磁共振成像（T1-MRI）、伊文思蓝注入法和组织学方法，在将细胞植入尾状壳核10天、16天和18天后，测量了啮齿动物脑肿瘤模型（RT2）中的颅内肿瘤体积。比较T1-MRI和伊文思蓝法测得的肿瘤体积之间（r2 = 0.99）、T1-MRI和组织学法测得的肿瘤体积之间（r2 = 0.98）以及组织学法和伊文思蓝法测得的肿瘤体积之间（r2 = 0.93）均具有良好的相关性。这些方法中的每一种在估计实验动物的肿瘤体积方面都是可靠的。肿瘤中钆和伊文思蓝有显著摄取，提示血脑屏障受到广泛破坏。
