Celma C, Allué J A, Pruñonosa J, Peraire C, Obach R
Metabolism and Pharmacokinetics Department, Ipsen Pharma, Sant Feliu de Llobregat (Barcelona), Spain.
J Chromatogr A. 2000 Feb 18;870(1-2):77-86. doi: 10.1016/s0021-9673(99)01252-2.
An analytical method for the determination of paracetamol and chlorpheniramine in human plasma has been developed, validated and applied to the analysis of samples from a phase I clinical trial. The analytical method consists in the extraction of paracetamol and chlorpheniramine with diethyl ether, followed by the determination of both drugs by an LC-MS-MS method, using 2-acetamidophenol as internal standard. The intra-assay and inter-assay precision and accuracy of this technique were good and the limit of quantitation was 0.5 microg/ml of plasma for paracetamol and 0.2 ng/ml for chlorpheniramine. The concentration working range was established between 0.5 microg/ml and 25 microg/ml for paracetamol and between 0.2 ng/ml and 50 ng/ml for chlorpheniramine. This method has been used for analyzing more than 1200 human plasma samples from a clinical study with 24 volunteers.
