采用高效液相色谱法测定大鼠皮肤渗透样品中酮洛芬的体外含量。

Determination by high-performance liquid chromatography of ketoprofen in vitro in rat skin permeation samples.

作者信息

de Jalón E G, Josa M, Campanero M A, Santoyo S, Ygartua P

机构信息

Centro Galénico, Departamento de Farmacia y Tecnología Farmacéutica, Universidad de Navarra, Pamplona, Spain.

出版信息

J Chromatogr A. 2000 Feb 18;870(1-2):143-9. doi: 10.1016/s0021-9673(99)00995-4.

DOI:10.1016/s0021-9673(99)00995-4

PMID:10722071

Abstract

A direct, simple and rapid high-performance liquid chromatographic method has been developed for the determination of ketoprofen with ibuprofen as internal standard. Samples were chromatographed on a 5 microm Kromasil 100 C18 column. The mobile phase was a mixture of acetonitrile-0.01 M KH2PO4 adjusted to pH 1.5 with orthophosphoric acid 85% (60:40, v/v). Detection was at 260 nm and the run time was 10 min. The detector response was found to be linear in the concentration range 0.02 to 40 microg/ml. This HPLC assay has been applied to measure the "in vitro" percutaneous penetration of ketoprofen through rat skin.

摘要

已开发出一种直接、简单且快速的高效液相色谱法，以布洛芬为内标物测定酮洛芬。样品在5微米的Kromasil 100 C18柱上进行色谱分析。流动相是乙腈与0.01M磷酸二氢钾的混合物，用85%的正磷酸调至pH 1.5（60:40，v/v）。检测波长为260nm，运行时间为10分钟。检测器响应在0.02至40微克/毫升的浓度范围内呈线性。该高效液相色谱法已应用于测定酮洛芬“体外”经大鼠皮肤的渗透情况。