Frankfater C, Maus E, Gaal K, Segade F, Copeland N G, Gilbert D J, Jenkins N A, Shipley J M
Department of Medicine, Barnes-Jewish Hospital, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Mamm Genome. 2000 Mar;11(3):191-5. doi: 10.1007/s003350010036.
A 1.4-kb EST clone encoding mouse microfibril-associated glycoprotein-2 (MAGP-2), identified by its similarity with the reported human cDNA, was used to screen a mouse 129 genomic bacterial artificial chromosome (BAC) library. The mouse gene contains 10 exons spanning 16 kb, located on the distal region of Chromosome (Chr) 6. The exons range in size from 24 to 963 bp, with the ATG located in exon 2. The tenth and largest exon contains 817 bp of 3' untranslated sequence, including a B2 repetitive element. Northern analysis demonstrates abundant expression of MAGP-2 mRNA in skeletal muscle, lung, and heart. Sequence analysis of additional cDNA clones suggests that the two mRNA forms of MAGP-2 in the mouse arise from alternative polyadenylation site usage. The promoter does not contain an obvious TATA box, and the sequence surrounding the start site does not conform to the consensus for an initiator promoter element. Additionally, the mouse promoter contains 22 copies of a CT dinucleotide repeat sequence located approximately 155 bp 5' to exon 1. MAGP-2 gene and compared it with that of the human gene (Hatzinikolas and Gibson 1998). While the mouse and human MAGP-2 proteins are similar in sequence, the promoters for the two genes share little in common. The presence of two mRNA species for MAGP-2 in the mouse raised the possibility that more than one isoform of the protein might be synthesized. We have characterized both mRNA species and determined that they do not code for different variants of the protein.
一个通过与已报道的人类cDNA序列相似性鉴定出的、编码小鼠微原纤维相关糖蛋白-2（MAGP-2）的1.4kb EST克隆，被用于筛选小鼠129基因组细菌人工染色体（BAC）文库。小鼠基因包含10个外显子，跨度为16kb，位于6号染色体（Chr）的远端区域。外显子大小从24bp到963bp不等，ATG位于外显子2中。第十个也是最大的外显子包含817bp的3'非翻译序列，包括一个B2重复元件。Northern分析表明MAGP-2 mRNA在骨骼肌、肺和心脏中大量表达。对其他cDNA克隆的序列分析表明，小鼠中MAGP-2的两种mRNA形式源于选择性聚腺苷酸化位点的使用。该启动子不包含明显的TATA盒，起始位点周围的序列不符合起始子启动子元件的共有序列。此外，小鼠启动子在距外显子1约155bp的5'端包含22个CT二核苷酸重复序列拷贝。对MAGP-2基因进行了表征，并将其与人类基因进行了比较（Hatzinikolas和Gibson，1998年）。虽然小鼠和人类的MAGP-2蛋白在序列上相似，但这两个基因的启动子几乎没有共同之处。小鼠中存在两种MAGP-2 mRNA物种，这增加了可能合成不止一种蛋白质异构体的可能性。我们对这两种mRNA物种进行了表征，并确定它们并不编码该蛋白质的不同变体。
