环糊精葡聚糖转移酶可降解马铃薯原淀粉的完整颗粒。

Cycloamylose (cyclodextrin) glucanotransferase degrades intact granules of potato raw starch.

作者信息

Yamamoto K, Zhang Z Z, Kobayashi S

机构信息

National Food Research Institute, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8642, Japan.

出版信息

J Agric Food Chem. 2000 Mar;48(3):962-6. doi: 10.1021/jf9905018.

DOI:10.1021/jf9905018

PMID:10725182

Abstract

Cycloamylose (cyclodextrin) glucanotransferase (EC 2.4.1.19, CGTase) originated from Bacillus macerans degraded intact granules of potato raw starch and converted them into cyclodextrins (CDs). The degradation required sufficient stirring of starch-CGTase suspension. The morphology of the degraded starch granules was unique; that is, the inner part of the granules was observed by scanning electron microscope to be more susceptible to CGTase than the outer part. Effects of pH, temperature, starch concentration, and enzyme amount on CD production were studied.

摘要

源自软化芽孢杆菌的环麦芽糊精（环糊精）葡糖基转移酶（EC 2.4.1.19，CGT酶）可降解完整的马铃薯生淀粉颗粒，并将其转化为环糊精（CDs）。这种降解需要对淀粉-CGT酶悬浮液进行充分搅拌。降解后的淀粉颗粒形态独特；也就是说，通过扫描电子显微镜观察发现，颗粒内部比外部更容易受到CGT酶的作用。研究了pH值、温度、淀粉浓度和酶量对CD产量的影响。