Department of Biochemistry, Centre for postgraduate studies. Jain University, Bengaluru, Karnataka, India.
School of Basic and Applied Sciences, Dayananda Sagar University, Bengaluru, India.
J Basic Microbiol. 2017 Nov;57(11):974-981. doi: 10.1002/jobm.201700270. Epub 2017 Aug 30.
Cyclomaltodextrin glucanotransferase is a unique enzyme that degrades starch into cyclic oligosaccharides called cyclodextrins, which have numerous applications in various industries such as pharmaceutical, textile, agricultural, cosmetics etc. Due to its wide applications, microorganism producing one type of cyclodextrin is of interest as it simplifies the down streaming process of separating mixture of cyclodextrins. In the present study, β-CGTase was isolated from Bacillus flexus SV 1 and biochemically characterized. Enzyme was purified by starch adsorption followed by DEAE cellulose column chromatography which resulted in a fold purification of 6.1, with a yield of 44.07%. Molecular weight of the purified enzyme was found to be 96.68 kDa, enzyme was monomeric in nature with a K and V of 0.08976 μmol mL and 585.1 μmol/ml/min, respectively. Optimum pH and temperature of the purified enzyme was found to be 8.0 and 60 °C. Ca showed significant increase in enzyme activity. The inhibition of enzyme by EDTA indicates that CGTase is a metalloenzyme. CGTase produced majorly β-CD and was alkalotolarent and active at high temperatures which is a promising candidate for various industries such as textile, food, agriculture, and pharmaceuticals.
环麦芽寡糖转糖苷酶是一种独特的酶，能够将淀粉降解为环状寡糖，称为环糊精，它在制药、纺织、农业、化妆品等各个行业有广泛的应用。由于其广泛的应用，微生物产生的一种环糊精引起了人们的兴趣，因为它简化了分离混合环糊精的下游处理过程。在本研究中，从柔韧芽孢杆菌 SV1 中分离出 β-CGTase，并对其进行了生化特性分析。通过淀粉吸附和 DEAE 纤维素柱层析对酶进行了纯化，酶的纯化倍数为 6.1，收率为 44.07%。纯化酶的分子量为 96.68 kDa，酶是单体，K 和 V 分别为 0.08976 μmol·mL 和 585.1 μmol/ml/min。纯化酶的最适 pH 和温度分别为 8.0 和 60°C。Ca 显著增加了酶的活性。EDTA 对酶的抑制表明 CGTase 是一种金属酶。CGTase 主要产生 β-CD，且对碱性和高温具有耐受性，是纺织、食品、农业和制药等各个行业有应用前景的候选酶。
