从序列到染色体：黑腹果蝇X染色体的末端

From sequence to chromosome: the tip of the X chromosome of D. melanogaster.

作者信息

Benos P V, Gatt M K, Ashburner M, Murphy L, Harris D, Barrell B, Ferraz C, Vidal S, Brun C, Demailles J, Cadieu E, Dreano S, Gloux S, Lelaure V, Mottier S, Galibert F, Borkova D, Minana B, Kafatos F C, Louis C, Sidén-Kiamos I, Bolshakov S, Papagiannakis G, Spanos L, Cox S, Madueño E, de Pablos B, Modolell J, Peter A, Schöttler P, Werner M, Mourkioti F, Beinert N, Dowe G, Schäfer U, Jäckle H, Bucheton A, Callister D M, Campbell L A, Darlamitsou A, Henderson N S, McMillan P J, Salles C, Tait E A, Valenti P, Saunder R D, Glover D M

机构信息

The European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton Hall, Cambridge CB10 1SD, UK.

出版信息

Science. 2000 Mar 24;287(5461):2220-2. doi: 10.1126/science.287.5461.2220.

DOI:10.1126/science.287.5461.2220

PMID:10731137

Abstract

One of the rewards of having a Drosophila melanogaster whole-genome sequence will be the potential to understand the molecular bases for structural features of chromosomes that have been a long-standing puzzle. Analysis of 2.6 megabases of sequence from the tip of the X chromosome of Drosophila identifies 273 genes. Cloned DNAs from the characteristic bulbous structure at the tip of the X chromosome in the region of the broad complex display an unusual pattern of in situ hybridization. Sequence analysis revealed that this region comprises 154 kilobases of DNA flanked by 1.2-kilobases of inverted repeats, each composed of a 350-base pair satellite related element. Thus, some aspects of chromosome structure appear to be revealed directly within the DNA sequence itself.

摘要

拥有黑腹果蝇全基因组序列的好处之一是，有可能了解长期以来一直是谜题的染色体结构特征的分子基础。对果蝇X染色体末端2.6兆碱基的序列分析鉴定出了273个基因。从宽泛复合体区域X染色体末端特征性的球茎状结构克隆的DNA显示出一种不寻常的原位杂交模式。序列分析表明，该区域由154千碱基的DNA组成，两侧是1.2千碱基的反向重复序列，每个反向重复序列由一个350碱基对的卫星相关元件组成。因此，染色体结构的某些方面似乎直接在DNA序列本身中得以揭示。