Losada A, Villasante A
Centro de Biología Molecular Severo Ochoa (CSIC-UAM).
Chromosome Res. 1996 Aug;4(5):372-83. doi: 10.1007/BF02257273.
During the screening of a Drosophila melanogaster YAC library with DNA from the minichromosome Dp(1;f)1187 we isolated a clone, yw20D5, which contains a new subtype of 1.688 satellite DNA. Although the sequences of several monomers subcloned from the YAC show a considerable variation in length, the derived consensus sequence is 356-bp long. This new subtype and the one constituted by the 353-bp repeats are both located on the left arm heterochromatin of chromosome 3, arranged in separate arrays. Despite their autosomal location, phylogenetic relationships among 1.688 satellite sequences suggest that they may have originated from the 359-bp repeats of the X chromosome heterochromatin. We have used the new 356-bp repeats to investigate whether sequences related to the 1.688 satellite are dispersed along the euchromatic arms of the autosomes in a similar way to that in which they are found along the X chromosome euchromatin.
在用来自小染色体Dp(1;f)1187的DNA筛选果蝇黑腹YAC文库的过程中，我们分离出了一个克隆，yw20D5，它包含一种新的1.688卫星DNA亚型。尽管从YAC亚克隆的几个单体的序列在长度上有相当大的差异，但推导的共有序列长356 bp。这种新亚型和由353 bp重复序列构成的亚型都位于3号染色体左臂异染色质上，排列成单独的阵列。尽管它们位于常染色体上，但1.688卫星序列之间的系统发育关系表明，它们可能起源于X染色体异染色质的359 bp重复序列。我们已经使用新的356 bp重复序列来研究与1.688卫星相关的序列是否以与在X染色体常染色质中发现的类似方式沿着常染色体的常染色质臂分散。
