Archbald L F, Tsai I F, Thatcher W W, Tran T, Wolfsdorf K, Risco C
Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32610, USA.
Theriogenology. 1998 Jun;49(8):1425-36. doi: 10.1016/s0093-691x(98)00089-2.
The objective of this study was to determine the value of using plasma concentrations of PGFM to diagnose subclinical endometritis in the dairy cow, and its relationship to subsequent fertility. A total of 274 cows between 24 to 29 d post partum was divided into 4 groups on the basis of clinical features of the uterus and ovary. Cows in Group 1 (n = 74) had a normal, involuting uterus and a CL on the ovary; cows in Group 2 (n = 51) had a normal, involuting uterus but no CL on the ovary; cows in Group 3 (n = 83) did not have a normal, involuting uterus but had a CL on the ovary; and cows in Group 4 (n = 66) did not have a normal, involuting uterus or a CL on the ovary. A blood sample was obtained from each cow on the day they were placed on the study, and plasma concentrations of PGFM and P4 were determined using RIA. Cows were artificially inseminated (AI) at the first observed estrus after Day 60 post partum, and pregnancy was determined by palpation of the uterus per rectum between 45 and 50 d postAI. Reproductive responses evaluated were conception rate to first service, days open, and percentage of cows pregnant by 90, 120, 150 and 180 d post partum. Data were analyzed using GLM procedures of SAS and a 2 x 2 factorial with contrast procedures. Polynomial regression analysis was used to determine the shape of the PGFM, P4 and fertility curves. There was no difference among mean PGFM concentrations of cows in each group. The rate of decline of plasma PGFM concentrations was lower in cows with an abnormal uterus and a CL on the ovary compared with those without a CL. A lower percentage of cows with abnormal uteri was pregnant by 90 d post partum compared with cows with normal uteri. From the results of this study, it was concluded that plasma PGFM concentrations between Days 24 to 29 post partum were not effective in identifying cows with subclinical endometritis.
本研究的目的是确定利用血浆中PGFM浓度诊断奶牛亚临床子宫内膜炎的价值及其与后续繁殖力的关系。总共274头产后24至29天的奶牛根据子宫和卵巢的临床特征被分为4组。第1组（n = 74）的奶牛子宫正常且正在 involuting，卵巢上有黄体（CL）；第2组（n = 51）的奶牛子宫正常且正在 involuting，但卵巢上没有CL；第3组（n = 83）的奶牛子宫不是正常且正在 involuting，但卵巢上有CL；第4组（n = 66）的奶牛子宫既不是正常且正在 involuting，卵巢上也没有CL。在将每头奶牛纳入研究当天采集血样，使用放射免疫分析法（RIA）测定血浆中PGFM和P4的浓度。奶牛在产后60天之后首次观察到发情时进行人工授精（AI），并在人工授精后45至50天通过直肠触诊子宫来确定是否怀孕。评估的繁殖反应包括首次输精的受胎率、空怀天数以及产后90、120、150和180天怀孕奶牛的百分比。数据使用SAS的GLM程序和带有对比程序的2×2析因分析进行分析。采用多项式回归分析来确定PGFM、P4和繁殖力曲线的形状。每组奶牛的平均PGFM浓度之间没有差异。与卵巢上没有CL的奶牛相比，子宫异常且卵巢上有CL的奶牛血浆PGFM浓度的下降速率更低。与子宫正常的奶牛相比，子宫异常的奶牛在产后90天怀孕的百分比更低。从本研究结果得出结论，产后24至29天的血浆PGFM浓度在识别患有亚临床子宫内膜炎的奶牛方面无效。
