Lechniak D, Cieślak D, Sosnowski J
Agricultural University of Poznań, Department of Genetics and Animal Breeding, Poland.
Theriogenology. 1998 Mar;49(4):779-85. doi: 10.1016/S0093-691X(98)00027-2.
We conducted a cytogenetic study of bovine parthenotes derived from oocytes matured and cultured in vitro. In vitro maturation was carried out by culturing follicular oocytes for 24 h in TCM199 supplemented with estrous cow serum (ECS) and hormones at 39 degrees C in 5% CO2. Matured oocytes were incubated for 20 h in sperm TALP without the addition of spermatozoa, after which they were cultured in maturation droplets for 48 to 72 h. Spontaneous activation occurred in 9.5% of the matured oocytes. Cytogenetic analysis of 24 parthenotes revealed that 62.5% exhibited a normal, diploid chromosome complement. The remaining 37.5% had various ploidy anomalies: haploidy (25%), triploidy (4.2%) and tetraploidy (8.3%). Parthenotes exhibited different developmental stages. The number of blastomeres ranged from 2 to 8 within a parthenote. Only 1 parthenote was comprised 9 to 16 cells. The results showed that spontaneous parthenogenetic activation which occurs in an IVM/IVF system may interfere with embryo production efficiency.
我们对源自体外成熟和培养的卵母细胞的牛孤雌胚胎进行了细胞遗传学研究。体外成熟是通过在补充有发情母牛血清（ECS）和激素的TCM199中于39℃、5%二氧化碳条件下培养卵泡卵母细胞24小时来进行的。将成熟的卵母细胞在不添加精子的精子TALP中孵育20小时，之后在成熟液滴中培养48至72小时。9.5%的成熟卵母细胞发生了自发激活。对24个孤雌胚胎的细胞遗传学分析显示，62.5%表现出正常的二倍体染色体组成。其余37.5%存在各种倍性异常：单倍体（25%）、三倍体（4.2%）和四倍体（8.3%）。孤雌胚胎表现出不同的发育阶段。一个孤雌胚胎内的卵裂球数量从2个到8个不等。只有1个孤雌胚胎由9至16个细胞组成。结果表明，在体外成熟/体外受精系统中发生的自发孤雌激活可能会干扰胚胎生产效率。
