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[Constructing of system of genetic analysis in Pseudomonas mendocina].

作者信息

Vasilenko S L, Titok M A, Maksimova N P

机构信息

Belorussian State University, Department of Genetics and Biotechnology, Minsk, Belarus.

出版信息

Genetika. 2000 Jan;36(1):28-33.

PMID:10732277
Abstract

A Tn10-containing variant of the pRK2013 plasmid, pRK2013-7, was used in the genetic analysis of Pseudomonas mendocina as chromosome-mobilizing inheritable factor that is able to integrate into the bacterial chromosome and transfer genetic markers with a frequency ranging from 3.2 x 10(-7) to 3.5 x 10(-3). The results of interrupted matings allowed localization of 10 genetic markers. This system of genetic analysis is suitable for P. mendocina mapping.

摘要

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