Høgdall E, Boye K, Vuust J
Department of Clinical Biochemistry, Statens Serum Institute, Copenhagen S, Denmark.
J Cell Biochem. 1999 Jun 15;73(4):433-6.
In an effort to find a simple and inexpensive purification method of polymerase chain reaction (PCR) reaction before cycle sequencing reaction, we compared a commercial system with a precipitation protocol performed in our laboratory. We found that, particularly with small PCR products, our method works with greater success than the method compared. Our precipitation method may be used on a larger PCR fragment before cycle sequencing reaction as well. Furthermore, it has the advantage of being simple as the well-known dilution method; in contrast to the dilution method, the precipitation method removes excess primers as well as possible primer dimers.
为了找到一种在循环测序反应前对聚合酶链反应（PCR）产物进行简单且低成本纯化的方法，我们将一种商业系统与我们实验室采用的沉淀方案进行了比较。我们发现，特别是对于小的PCR产物，我们的方法比所比较的方法成功率更高。我们的沉淀方法也可用于循环测序反应前更大的PCR片段。此外，它具有与著名的稀释法一样简单的优点；与稀释法不同的是，沉淀法能去除过量引物以及可能存在的引物二聚体。
