Misra A K, Rao M M, Kasiraj R, Reddy N S, Pant H C
Sabarmati Ashram Gaushala, Kaira, India.
Theriogenology. 1999 Sep;52(4):701-7. doi: 10.1016/s0093-691x(99)00163-6.
This study was conducted to compare fertilization rate and viable embryo recovery rate in superovulated buffalo (n = 64) following insemination with semen from buffalo bulls (n = 5) having different fertility rates as determined by AI. Frozen-thawed semen from fertile bulls with similar post-thaw progressive motility and sperm morphology was used to inseminate buffalo at superovulatory estrus. Fertilization and viable embryo recovery rates differed among bulls, but this bull-specific effect was not related to the overall herd fertility rate as determined by AI in normal cyclic animals. These results indicate that individual bulls differ in their contribution to fertilization of superovulated donors and also to embryonic development, as determined by viable embryo recovery. Moreover, the results also suggest that buffalo bulls can be screened for optimal fertility and embryo recovery rates in superovulated donors. Further studies are warranted to ascertain the factors which contribute to such bull-specific effects.
本研究旨在比较经超数排卵的水牛（n = 64）在人工授精后，使用不同受胎率的水牛公牛（n = 5）精液受精率和活胚回收率的差异，这些公牛的受胎率由人工授精确定。使用解冻后具有相似前进运动性和精子形态的可育公牛冷冻精液，在超数排卵发情期对水牛进行人工授精。公牛之间的受精率和活胚回收率存在差异，但这种公牛特异性效应与正常发情周期动物人工授精所确定的总体群体受胎率无关。这些结果表明，个体公牛在对超数排卵供体的受精以及胚胎发育（通过活胚回收率确定）的贡献方面存在差异。此外，结果还表明，可以筛选水牛公牛，以获得超数排卵供体的最佳受胎率和胚胎回收率。有必要进行进一步研究以确定导致这种公牛特异性效应的因素。
